Patarroyo M, Beatty P G, Fabre J W, Gahmberg C G
Scand J Immunol. 1985 Aug;22(2):171-82. doi: 10.1111/j.1365-3083.1985.tb01869.x.
Phorbol esters rapidly induce aggregation of human mononuclear leukocytes in vitro. Previous studies have indicated that cell surface proteins are involved. We report now that the monoclonal antibody 60.3, either as purified IgG or as Fab' fragments, to an antigen common to leukocytes completely inhibited the phorbol ester-induced intercellular adhesion (binding). No inhibition of cell aggregation was observed with monoclonal antibodies to common leukocyte antigen T 200, T-cell-associated antigen, monocyte-granulocyte antigen, brain granulocyte-T-lymphocyte antigen, transferrin receptor, mature T-cell antigens (mol.wt either 67,000 or 19,000/29,000), T helper/inducer cell antigen, sheep erythrocyte receptor, class I or class II antigens, or T cytotoxic/suppressor cell antigen. The antibody 60.3 did not inhibit stimulation of the cells since the characteristic phorbol ester-induced morphological changes and phorbol ester-enhanced cap formation of membrane glycoproteins were readily observed. Two major cell surface polypeptides with apparent molecular weights of 90,000 and 160,000 were immunoprecipitated. We conclude that this protein complex, or at least one of its components, mediates adhesion among mononuclear leukocytes.
佛波酯能在体外迅速诱导人单核白细胞聚集。先前的研究表明细胞表面蛋白参与其中。我们现在报告，针对白细胞共同抗原的单克隆抗体60.3，无论是纯化的IgG还是Fab'片段，都能完全抑制佛波酯诱导的细胞间黏附（结合）。针对常见白细胞抗原T 200、T细胞相关抗原、单核细胞-粒细胞抗原、脑粒细胞-T淋巴细胞抗原、转铁蛋白受体、成熟T细胞抗原（分子量分别为67,000或19,000/29,000）、T辅助/诱导细胞抗原、绵羊红细胞受体、I类或II类抗原，或T细胞毒性/抑制细胞抗原的单克隆抗体均未观察到对细胞聚集的抑制作用。抗体60.3并未抑制细胞的刺激，因为很容易观察到典型的佛波酯诱导的形态变化以及佛波酯增强的膜糖蛋白帽形成。免疫沉淀出了两条表观分子量分别为90,000和160,000的主要细胞表面多肽。我们得出结论，这种蛋白复合物，或至少其一种成分，介导单核白细胞之间的黏附。
