The First Affiliated Hospital of Bengbu Medical University, Bengbu Medical University, Bengbu, Anhui Province, 233044, People's Republic of China.
Int J Nanomedicine. 2024 Jun 25;19:6359-6376. doi: 10.2147/IJN.S461996. eCollection 2024.
Bone tissue engineering (BTE) is a promising alternative to autologous bone grafting for the clinical treatment of bone defects, and inorganic/organic composite hydrogels as BTE scaffolds are a hot spot in current research. The construction of nano-hydroxyapatite/gelatin methacrylate/oxidized sodium alginate (nHAP/GelMA/OSA), abbreviated as HGO, composite hydrogels loaded with bone morphogenetic protein 7 (BMP7) will provide a suitable 3D microenvironment to promote cell aggregation, proliferation, and differentiation, thus facilitating bone repair and regeneration.
Dually-crosslinked hydrogels were fabricated by combining GelMA and OSA, while HGO hydrogels were formulated by incorporating varying amounts of nHAP. The hydrogels were physically and chemically characterized followed by the assessment of their biocompatibility. BMP7-HGO (BHGO) hydrogels were fabricated by incorporating suitable concentrations of BMP7 into HGO hydrogels. The osteogenic potential of BHGO hydrogels was then validated through in vitro experiments and using rat femoral defect models.
The addition of nHAP significantly improved the physical properties of the hydrogel, and the composite hydrogel with 10% nHAP demonstrated the best overall performance among all groups. The selected concentration of HGO hydrogel served as a carrier for BMP7 loading and was evaluated for its osteogenic potential both in vivo and in vitro. The BHGO hydrogel demonstrated superior in vitro osteogenic induction and in vivo potential for repairing bone tissue compared to the outcomes observed in the blank control, BMP7, and HGO groups.
Using hydrogel containing 10% HGO appears promising for bone tissue engineering scaffolds, especially when loaded with BMP7 to boost its osteogenic potential. However, further investigation is needed to optimize the GelMA, OSA, and nHAP ratios, along with the BMP7 concentration, to maximize the osteogenic potential.
骨组织工程（BTE）是自体骨移植治疗骨缺损的一种有前途的替代方法，无机/有机复合水凝胶作为 BTE 支架是当前研究的热点。构建纳米羟基磷灰石/甲基丙烯酰化明胶/氧化海藻酸钠（nHAP/GelMA/OSA），简称 HGO，复合水凝胶负载骨形态发生蛋白 7（BMP7）将提供合适的 3D 微环境，促进细胞聚集、增殖和分化，从而促进骨修复和再生。
通过将 GelMA 和 OSA 结合来制备双重交联水凝胶，同时通过掺入不同量的 nHAP 来制备 HGO 水凝胶。对水凝胶进行物理化学特性表征，然后评估其生物相容性。通过将适当浓度的 BMP7 掺入 HGO 水凝胶中制备 BMP7-HGO（BHGO）水凝胶。然后通过体外实验和大鼠股骨缺损模型验证 BHGO 水凝胶的成骨潜力。
添加 nHAP 显著改善了水凝胶的物理性能，在所有组中，含有 10%nHAP 的复合水凝胶表现出最佳的综合性能。选择的 HGO 水凝胶浓度作为 BMP7 负载的载体，并在体内和体外评估其成骨潜力。与空白对照、BMP7 和 HGO 组相比，BHGO 水凝胶在体外具有更好的成骨诱导能力，在体内具有修复骨组织的潜力。
使用含有 10%HGO 的水凝胶作为骨组织工程支架具有很大的前景，特别是在负载 BMP7 以增强其成骨潜力的情况下。然而，需要进一步研究来优化 GelMA、OSA 和 nHAP 的比例以及 BMP7 的浓度，以最大限度地发挥其成骨潜力。
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