临床、分子和血清学特征在儿童细小病毒 B19 感染诊断中的作用。
Role of clinical, molecular, and serological features in the diagnosis of parvovirus B19 infection in children.
机构信息
Department of Medical Microbiology, Istanbul University, Istanbul Faculty of Medicine, Istanbul, Turkey.
Department of Pediatrics, Istanbul University, Istanbul Faculty of Medicine, Istanbul, Turkey.
出版信息
Diagn Microbiol Infect Dis. 2024 Aug;109(4):116300. doi: 10.1016/j.diagmicrobio.2024.116300. Epub 2024 Apr 18.
BACKGROUND
Parvovirus B19(B19) is a DNA virus. The most common B19 disease is erythema infectiosum (fifth-disease). PCR and ELISA are sensitive for detecting of acute disease. However, it is not clear which test better and the relationship between laboratory tests and clinical findings.
OBJECTIVE
To discuss the clinical and laboratory characteristics of pediatric patients infected with B19.
STUDY DESIGN
236 children were examined. Children with at least one positive molecular or serological test were included. Positive serum B19-DNA and/or B19-IgM was considered an acute B19 infection.
RESULTS
B19DNA was detected in 80.8 % of acute cases. Serological tests were less positive. Acute B19 infection was observed in 24 patients. Only 17 patients were positive for B19 DNA, 3 for IgM and 4 for both. The sensitivity of B19 DNA is 87.5 %. However, this rate is 29.2 % for B19 IgM.
CONCLUSION
B19-DNA and IgM together provide a better, highly accurate diagnosis.
背景
细小病毒 B19（B19）是一种 DNA 病毒。最常见的 B19 疾病是红斑疹（第五病）。PCR 和 ELISA 对于检测急性疾病非常敏感。然而，目前尚不清楚哪种检测方法更好，以及实验室检测与临床发现之间的关系。
目的
探讨儿童感染 B19 的临床和实验室特征。
研究设计
检查了 236 名儿童。至少有一种分子或血清学检测呈阳性的儿童被纳入研究。阳性血清 B19-DNA 和/或 B19-IgM 被认为是急性 B19 感染。
结果
80.8%的急性病例中检测到 B19DNA。血清学检测的阳性率较低。24 例患儿出现急性 B19 感染。仅 17 例患者 B19 DNA 阳性，3 例 IgM 阳性，4 例两者均阳性。B19 DNA 的灵敏度为 87.5%。然而，B19 IgM 的阳性率仅为 29.2%。
结论
B19-DNA 和 IgM 联合检测可提供更好、更准确的诊断。
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