Jensen O L, Gluud B S, Eriksen H O
Acta Ophthalmol (Copenh). 1985 Jun;63(3):346-50. doi: 10.1111/j.1755-3768.1985.tb06818.x.
The concentration of the high molecular glycoprotein fibronectin (FN) was measured by an enzyme-linked immunoadsorbent technique (ELISA) in tears of 25 patients before and after cataract extraction. Both native and degraded FN was measured. The median concentration of FN in tears before surgery was 0.28 mg/l (range: 0.1-3.8 mg/l) and 0.18 mg/l (range: 0.1-1.95 mg/l for degraded and native FN, respectively. The time course of the median values of both types of FN during the post-operative observation period described a biphasic curve with peaks at the second and fifth day following operation. On the twelfth day the concentration of degraded and native FN had almost reached pre-operative levels. The variation in the post-operative FN concentration proved statistically significant. The biological significance of the changes in FN level in the post-operative period is not obvious; the first peak may be explained by a spill-over from plasma, and the second peak may reflect FN production from fibroblasts in the granulation tissue of the corneoscleral cicatrix.
采用酶联免疫吸附技术（ELISA）对25例患者白内障摘除术前、后的泪液中高分子糖蛋白纤连蛋白（FN）浓度进行了检测。同时检测了天然型和降解型FN。术前泪液中FN的中位数浓度为0.28mg/L（范围：0.1 - 3.8mg/L），降解型和天然型FN分别为0.18mg/L（范围：0.1 - 1.95mg/L）。术后观察期内两种类型FN中位数的时间变化过程呈双相曲线，在术后第2天和第5天出现峰值。术后第12天，降解型和天然型FN的浓度几乎恢复到术前水平。术后FN浓度的变化具有统计学意义。术后FN水平变化的生物学意义尚不明确；第一个峰值可能是血浆溢出所致，第二个峰值可能反映了角膜巩膜瘢痕肉芽组织中纤维母细胞产生的FN。
