三代 PacBio 测序技术探索结直肠癌中的肠道细菌和性别差异。

Third-generation PacBio sequencing to explore gut bacteria and gender in colorectal cancer.

机构信息

Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, Zhejiang Province, People's Republic of China; Fifth School of Clinical Medicine of Zhejiang Chinese Medical University (Huzhou Central Hospital), Zhejiang Province, People's Republic of China; Key Laboratory of Multiomics Research and Clinical Transformation of Digestive Cancer of Huzhou, Zhejiang Province, People's Republic of China.

出版信息

Microb Pathog. 2024 Jul;192:106684. doi: 10.1016/j.micpath.2024.106684. Epub 2024 May 15.

DOI:10.1016/j.micpath.2024.106684

PMID:38759934

Abstract

BACKGROUND

Gut bacteria have an important influence on colorectal cancer (CRC). The differences of gut bacteria between genders have been the hot spots.

OBJECTIVE

To analyze the relationship between gut bacteria and gender differences in patients with CRC.

METHODS

A total of 212 patients with CRC and 212 healthy volunteers were recruited. The subjects' fecal samples were obtained, and the fecal microorganisms were analyzed by the third-generation sequencing PacBio. The composition of gut bacteria was analyzed. Linear discriminant analysis Effect Size (LEfSe) was used to analyze the differences in gut bacteria. Pearson coefficient was used to calculate the correlation between differential bacteria. CRC risk prediction models were used to rank the importance of effective differential bacteria.

RESULTS

Escherichia flexneri and Phocaeicola vulgatus were the most frequent bacteria in both male and female CRC patients. Bacteroides, Verrucomicrobia and Akkermansiaceae were highly enriched in male CRC group, while Bacteroidetes, Phocaeicola and Tissierellales were highly enriched in female CRC group. Peptostreptococcus anaerobius and Phocaeicola vulgatus were important CRC related bacteria in males and females, respectively. Peptostreptococcus anaerobius was the most important characteristic bacterium of males (AUC = 0.951), and the sensitivity and specificity of the discovery set were 78.74 % and 93.98 %, respectively. Blautia stercoris was the most important characteristic bacterium of females (AUC = 0.966), and the sensitivity and specificity of the discovery set were 90.63 % and 90.63 %, respectively.

CONCLUSION

Gut bacteria varied in different genders. Therefore, gender should be considered when gut bacteria are applied in the diagnose and prevention of CRC.

摘要

背景

肠道细菌对结直肠癌（CRC）有重要影响。肠道细菌在性别之间的差异一直是热点。

目的

分析 CRC 患者肠道细菌与性别差异的关系。

方法

共纳入 212 例 CRC 患者和 212 例健康志愿者。采集受试者粪便样本，采用第三代测序 PacBio 分析粪便微生物。分析肠道细菌组成。采用线性判别分析效应量（LEfSe）分析肠道细菌差异。用 Pearson 系数计算差异细菌之间的相关性。使用 CRC 风险预测模型对有效差异细菌的重要性进行排序。

结果

在男性和女性 CRC 患者中，最常见的细菌是大肠杆菌和 Phocaeicola vulgatus。拟杆菌、疣微菌科和阿克曼氏菌科在男性 CRC 组中高度富集，而厚壁菌门、Phocaeicola 和 Tissierellales 在女性 CRC 组中高度富集。消化链球菌和 Phocaeicola vulgatus 分别是男性和女性 CRC 的重要相关细菌。消化链球菌是男性最具特征性的细菌（AUC=0.951），发现集的灵敏度和特异性分别为 78.74%和 93.98%。Blautia stercoris 是女性最具特征性的细菌（AUC=0.966），发现集的灵敏度和特异性均为 90.63%。