College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Hunan Agricultural Products Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Acta Biochim Biophys Sin (Shanghai). 2024 May 16;56(9):1300-1310. doi: 10.3724/abbs.2024042.
Lotus seed skin extract is rich in flavonoids, making it a promising candidate for developing health products. In a previous study, we found that proanthocyanidins from lotus seed skin, particularly proanthocyanidin B1 (PB1), can indirectly activate the Nrf2 signaling pathway, exerting an antioxidant effect. In this study, we isolate proanthocyanidins from lotus seed skin (PLS) using ethanol extraction and RP-HPLC identification, and investigate its effects on glycolipid metabolism both and . Our results demonstrate that PLS reduces body weight in high-fat diet (HFD) mice by decreasing feed efficiency. PLS also normalizes serum glucose, insulin secretion, glycosylated hemoglobin (HbA1c), and intraperitoneal glucose tolerance (IPGTT). Furthermore, PLS significantly improves blood lipid parameters and inhibits the expressions of six proinflammatory factors, including IL-1α, IL-1β, IL-3, IL-6, IFN-γ and TNF-α in HFD mice. Additionally, analysis of fresh liver tissues reveals that PLS and PB1 induce the expressions of antioxidant proteins such as HO-1 and NQO1 by activating the p38-Nrf2 signaling pathway and inhibiting the NF-κB signaling pathway. In conclusion, proanthocyanidins from lotus seed skin regulate glycolipid metabolism disorders by targeting the p38/Nrf2/NF-κB signaling pathway. Our study offers a new approach for the high-value comprehensive utilization of lotus seed skin by-products and precise dietary intervention for metabolic syndrome.
莲子皮提取物富含类黄酮，是开发保健品的有前途的候选物。在之前的研究中，我们发现莲子皮中原花青素，特别是原花青素 B1（PB1），可以间接激活 Nrf2 信号通路，发挥抗氧化作用。在这项研究中，我们使用乙醇提取和 RP-HPLC 鉴定从莲子皮中分离原花青素（PLS），并研究其对糖脂代谢的影响。我们的结果表明，PLS 通过降低饲料效率来降低高脂肪饮食（HFD）小鼠的体重。PLS 还使血清葡萄糖、胰岛素分泌、糖化血红蛋白（HbA1c）和腹腔内葡萄糖耐量（IPGTT）正常化。此外，PLS 显著改善血脂参数，并抑制 HFD 小鼠六种促炎因子（包括 IL-1α、IL-1β、IL-3、IL-6、IFN-γ 和 TNF-α）的表达。此外，对新鲜肝组织的分析表明，PLS 和 PB1 通过激活 p38-Nrf2 信号通路和抑制 NF-κB 信号通路，诱导 HO-1 和 NQO1 等抗氧化蛋白的表达。总之，莲子皮中原花青素通过靶向 p38/Nrf2/NF-κB 信号通路调节糖脂代谢紊乱。我们的研究为莲子皮副产物的高附加值综合利用和代谢综合征的精确饮食干预提供了新的方法。
