Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
National Key Laboratory of Agricultural Microbiology, Angel Yeast Co., Ltd., Yichang 443003, PR China; The Hubei Provincial Key Laboratory of Yeast Function, Angel Yeast Co., Ltd., Yichang 443003, PR China; Yi Chang Engineering and Technology Research Center of Nutrition and Health Food, Angel Yeast Co., Ltd., Yichang 443003, PR China.
Microbiol Res. 2024 Aug;285:127741. doi: 10.1016/j.micres.2024.127741. Epub 2024 May 9.
Fructooligosaccharides (FOS) are a common prebiotic widely used in functional foods. Meanwhile, Saccharomyces boulardii is a fungal probiotic frequenly used in the clinical treatment of diarrhea. Compared with single use, the combination of prebiotics and probiotics as symbiotics may be more effective in regulating gut microbiota as recently reported in the literature. The present study aimed to investigate the effects of FOS, S. boulardii and their combination on the structure and metabolism of the gut microbiota in healthy primary and secondary school students using an in vitro fermentation model. The results indicated that S. boulardii alone could not effectively regulate the community structure and metabolism of the microbiota. However, both FOS and the combination of FOS and S. boulardii could effectively regulate the microbiota, significantly inhibiting the growth of Escherichia-Shigella and Bacteroides, and controlling the production of the gases including HS and NH. In addition, both FOS and the combination could significantly promote the growth of Bifidobacteria and Lactobacillus, lower environmental pH, and enhance several physiological functions related to synthesis and metabolism. Nevertheless, the combination had more unique benefits as it promoted the growth of Lactobacillus, significantly increased CO production and enhanced the functional pathways of carbon metabolism and pyruvic acid metabolism. These findings provide guidance for clinical application and a theoretical basis for the development of synbiotic preparations.
低聚果糖（FOS）是一种常用的益生元，广泛应用于功能性食品中。同时，布拉氏酵母菌是一种真菌益生菌，常用于临床腹泻的治疗。与单一使用相比，益生元和益生菌的组合作为共生体可能更有效地调节肠道微生物群，这一点最近在文献中有所报道。本研究旨在使用体外发酵模型，研究 FOS、布拉氏酵母菌及其组合对健康中小学生肠道微生物群结构和代谢的影响。结果表明，单独使用布拉氏酵母菌不能有效调节微生物群的群落结构和代谢。然而，FOS 和 FOS 与布拉氏酵母菌的组合都能有效地调节微生物群，显著抑制大肠杆菌-志贺氏菌和拟杆菌的生长，并控制包括 HS 和 NH 在内的气体的产生。此外，FOS 和组合都能显著促进双歧杆菌和乳酸杆菌的生长，降低环境 pH 值，并增强与合成和代谢相关的几种生理功能。然而，组合具有更独特的益处，因为它促进了乳酸杆菌的生长，显著增加了 CO 的产生，并增强了碳代谢和丙酮酸代谢的功能途径。这些发现为临床应用提供了指导，并为共生制剂的开发提供了理论依据。
