College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Longhu Laboratory of Advanced Immunology, Zhengzhou 450046, China; School of Advanced Agriculture Sciences, Peking University, Beijing 100871, China.
Int J Biol Macromol. 2024 Jun;270(Pt 1):132432. doi: 10.1016/j.ijbiomac.2024.132432. Epub 2024 May 17.
The African swine fever virus (ASFV) continues to pose significant economic and pandemic risks. Consequently, discovering new, efficient vaccines is crucial. Messenger RNA (mRNA) vaccines have emerged as promising candidates, providing minimal risk of insertional mutagenesis, high safety profiles, effectiveness, rapid scalability in production, and cost-effectiveness. In this study, we have developed an ASF p30 mRNA vaccine candidate (mRNA/Man-LNP) employing mannose-modified lipid nanoparticles (LNPs). The mRNA/Man-LNP exhibited effective antigen presentation and facilitated dendritic cells (DCs) maturation. Notably, it elicited strong IgG titers and activated CD4 and CD8 T-cells in immunized mice, all while adhering to stringent biosafety standards. This investigation demonstrates that mRNA/Man-LNP can trigger both humoral and cellular immune responses, suggesting its potential as a potent and promising vaccine candidate for controlling African swine fever (ASF).
非洲猪瘟病毒（ASFV）继续构成重大的经济和大流行风险。因此，发现新的、有效的疫苗至关重要。信使 RNA（mRNA）疫苗作为一种很有前途的候选物，具有最小的插入突变风险、高安全性、有效性、在生产中快速可扩展性和成本效益。在这项研究中，我们开发了一种非洲猪瘟 p30 mRNA 疫苗候选物（mRNA/Man-LNP），采用了甘露糖修饰的脂质纳米颗粒（LNPs）。mRNA/Man-LNP 表现出有效的抗原呈递作用，并促进树突状细胞（DC）成熟。值得注意的是，它在免疫小鼠中引发了强烈的 IgG 滴度，并激活了 CD4 和 CD8 T 细胞，同时符合严格的生物安全标准。这项研究表明，mRNA/Man-LNP 可以引发体液和细胞免疫反应，这表明它有可能成为控制非洲猪瘟（ASF）的一种有效且有前途的疫苗候选物。
