Nordlind K, Thyberg J
Int Arch Allergy Appl Immunol. 1983;71(4):363-7. doi: 10.1159/000233421.
In vitro effects of the retinoids 13-cis-retinoic acid (Ro4-3780) and etretinate (Ro 10-9359) on DNA synthesis and the fine structure of different guinea pig and human lymphoid cells were tested. 13-Cis-retinoic acid caused a dose-dependent inhibition of DNA synthesis most prominently in thymocytes. This effect was not due to an inhibition of thymidine uptake into the cells. Electron microscopically, the cells exhibited swollen Golgi cisternae, enlarged mitochondria often with condensed matrix, and an increased number of autophagic vacuoles. Etretinate, on the other hand, was without effect on DNA synthesis as well as on cell fine structure. The results suggest that the morphological and growth-inhibitory effects of 13-cis-retinoic acid are related.
测试了维甲酸13 - 顺式维甲酸（Ro4 - 3780）和阿维A酯（Ro 10 - 9359）对不同豚鼠和人类淋巴细胞DNA合成及精细结构的体外作用。13 - 顺式维甲酸对DNA合成产生剂量依赖性抑制，在胸腺细胞中最为显著。这种作用并非由于细胞对胸苷摄取的抑制。在电子显微镜下，细胞呈现高尔基体池肿胀、线粒体增大且基质常浓缩，以及自噬泡数量增加。另一方面，阿维A酯对DNA合成以及细胞精细结构均无影响。结果表明，13 - 顺式维甲酸的形态学和生长抑制作用是相关的。
