主要组织相容性复合体I类同种异体抗原的碳水化合物部分并非T淋巴细胞识别它们所必需的。
Carbohydrate moieties of major histocompatibility complex class I alloantigens are not required for their recognition by T lymphocytes.
作者信息
Goldstein S A, Mescher M F
出版信息
J Exp Med. 1985 Oct 1;162(4):1381-6. doi: 10.1084/jem.162.4.1381.
Abstract
The ability to generate specific cytotoxic responses using purified major histocompatibility complex (MHC) antigen in liposomes has made it possible to directly assess the importance of class I carbohydrate moieties in T cell recognition of alloantigen. Deglycosylation of affinity-purified H-2Kk to yield a single glycan-free product did not alter the specificity, the magnitude, nor the dose range of the cytotoxic T lymphocyte (CTL) response to the class I antigen. It can be concluded that carbohydrate moieties are not required to maintain the necessary conformation of the MHC protein, nor to interact with either the antigen-specific receptor or accessory proteins on precursor CTL.
摘要
利用脂质体中的纯化主要组织相容性复合体（MHC）抗原产生特异性细胞毒性反应的能力，使得直接评估I类碳水化合物部分在T细胞识别同种异体抗原中的重要性成为可能。对亲和纯化的H-2Kk进行去糖基化，得到单一无糖基产物，这并未改变细胞毒性T淋巴细胞（CTL）对I类抗原反应的特异性、强度或剂量范围。可以得出结论，碳水化合物部分对于维持MHC蛋白的必要构象不是必需的，对于与前体CTL上的抗原特异性受体或辅助蛋白相互作用也不是必需的。
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