Department of Dental Biomaterials, Faculty of Dentistry, Mansoura University, Mansoura City, Egypt.
Department of Conservative Dentistry, Faculty of Dentistry, Mansoura University, Mansoura City, Egypt.
BMC Oral Health. 2024 May 19;24(1):581. doi: 10.1186/s12903-024-04304-8.
This study was conducted to compare chemical, elemental and surface properties of sound and carious dentin after application of two restorative materials resin-modified glassionomer claimed to be bioactive and glass hybrid restorative material after enzymatic chemomechanical caries removal (CMCR) agent.
Forty carious and twenty non-carious human permanent molars were used. Molars were randomly distributed into three main groups: Group 1 (negative control) - sound molars, Group 2 (positive control) - molars were left without caries removal and Group 3 (Test Group) caries excavated with enzymatic based CMCR agent. After caries excavation and restoration application, all specimens were prepared Vickers microhardness test (VHN), for elemental analysis using Energy Dispersive Xray (EDX) mapping and finally chemical analysis using Micro-Raman microscopy.
Vickers microhardness values of dentin with the claimed bioactive GIC specimens was statistically higher than with glass hybrid GIC specimens. EDX analysis at the junction estimated: Calcium and Phosphorus of the glass hybrid GIC showed insignificantly higher mean valued than that of the bioactive GIC. Silica and Aluminum mean values at the junction were significantly higher with bioactive GIC specimens than glass hybrid GIC specimen. Micro-raman spectroscopy revealed that bioactive GIC specimens showed higher frequencies of v PO which indicated high level of remineralization.
It was concluded that ion-releasing bioactive resin-based restorative material had increased the microhardness and remineralization rate of carries affected and sound dentin. In addition, enzymatic caries excavation with papain-based CMCR agent has no adverse effect on dentin substrate.
本研究旨在比较两种声称具有生物活性的修复材料——树脂改性玻璃离子体和玻璃混合修复材料——在应用于经酶化学机械龋去腐（CMCR）剂处理后的正常和龋坏牙本质后的化学、元素和表面性质。
使用 40 颗龋坏和 20 颗非龋坏的人恒磨牙。磨牙随机分为三组：第 1 组（阴性对照）-正常磨牙，第 2 组（阳性对照）-不进行龋去腐处理，第 3 组（实验组）-用酶基 CMCR 剂进行龋蚀。龋蚀去除和修复材料应用后，所有标本均进行维氏硬度测试（VHN）、能量色散 X 射线（EDX）mapping 元素分析和微拉曼显微镜化学分析。
声称具有生物活性的玻璃离子体试件的牙本质维氏硬度值明显高于玻璃混合玻璃离子体试件。交界处的 EDX 分析估计：玻璃混合玻璃离子体的钙和磷平均值略高于生物活性玻璃离子体。交界处的硅和铝平均值明显高于生物活性玻璃离子体试件。微拉曼光谱显示，生物活性玻璃离子体试件显示出更高的 vPO 频率，表明再矿化程度较高。
离子释放型生物活性树脂基修复材料可提高龋坏和正常牙本质的显微硬度和再矿化速度。此外，基于木瓜蛋白酶的 CMCR 剂的酶化学龋去腐处理对牙本质基质无不良影响。
