Inazawa M, Shiozawa C, Fotedar R, Diener E
Lymphokine Res. 1985 Fall;4(4):343-50.
For the purpose of arriving at a unifying concept concerning the mechanisms in control of B lymphocyte responses, LPS or anti-lg receptor antibodies have conveniently served as substitutes for antigen. We report that the proliferative B cell responses in serum protein free medium to each of these polyclonal activators differ in the requirement for B cell growth factors (BCGF). Murine B lymphocytes which were prestimulated with anti-lg (Fab')2 antibody could readily be induced by semipurified BCGF (containing some IL2 activity) to incorporate thymidine. In contrast, B lymphocytes which were prestimulated with LPS failed to respond to BCGF, but could be restimulated by LPS. We have also shown that the dependence on B cell maturation factors of antigen affinity enriched B lymphocytes to develop into antibody forming cells (AFC) in response to LPS, depends on the antigen for which they were selected. B cells that have been affinity enriched with chicken red blood cells (CRBC) and stimulated by LPS to proliferate require maturation factors in order to generate a CRBC specific IgM response. In contrast, B cells that have been affinity enriched for TNP may be induced by LPS alone to generate TNP specific AFC independent of maturation factors. The results question the general validity of theoretical concepts concerning the role of lymphokines in B cell triggering, where such concepts are derived from experiments with LPS-activated B cells.
为了得出关于控制B淋巴细胞反应机制的统一概念，脂多糖（LPS）或抗Ig受体抗体已被方便地用作抗原的替代物。我们报告，在无血清蛋白培养基中，B细胞对这些多克隆激活剂各自的增殖反应对B细胞生长因子（BCGF）的需求不同。用抗Ig（Fab'）2抗体预刺激的小鼠B淋巴细胞很容易被半纯化的BCGF（含有一些白细胞介素2活性）诱导掺入胸苷。相比之下，用LPS预刺激的B淋巴细胞对BCGF无反应，但可被LPS再次刺激。我们还表明，抗原亲和力富集的B淋巴细胞在响应LPS时发育成抗体形成细胞（AFC）对B细胞成熟因子的依赖性，取决于它们所针对的抗原。用鸡红细胞（CRBC）进行亲和力富集并被LPS刺激增殖的B细胞，需要成熟因子才能产生CRBC特异性IgM反应。相比之下，对三硝基苯（TNP）进行亲和力富集的B细胞可能仅被LPS诱导产生TNP特异性AFC，而不依赖于成熟因子。这些结果质疑了关于淋巴因子在B细胞触发中作用的理论概念的普遍有效性，这些概念是从LPS激活的B细胞实验中得出的。
