Müller W, Kühn R, Goldmann W, Tesch H, Smith F I, Radbruch A, Rajewsky K
J Immunol. 1985 Aug;135(2):1213-9.
Mouse B lymphocytes were stimulated at high cell concentrations with goat anti-IgM antibodies, which leads to the induction of B cell proliferation without the addition of any growth factors. After 48 hr, blast cells were purified and cultured at low cell concentrations. Proliferation and differentiation of purified B lymphocyte blasts is then dependent on the addition of either mitogens (e.g., LPS) or certain lymphokines derived from activated T cells or macrophages. One such lymphokine was isolated from supernatants of various activated T cells and characterized by gel filtration as a material with an apparent m.w. of 40,000 to 50,000, similar to BCGF II. It supports the proliferation of the B cell blasts and induces their differentiation into plaque-forming cells. Lymphokines such as BCGF I, interleukin 2, and BCDF gamma could neither maintain growth nor induce differentiation of B lymphocytes preactivated by goat anti-IgM.
用山羊抗 IgM 抗体在高细胞浓度下刺激小鼠 B 淋巴细胞，这会在不添加任何生长因子的情况下诱导 B 细胞增殖。48 小时后，纯化母细胞并在低细胞浓度下培养。纯化的 B 淋巴细胞母细胞的增殖和分化随后取决于添加丝裂原（如脂多糖）或某些源自活化 T 细胞或巨噬细胞的淋巴因子。从各种活化 T 细胞的上清液中分离出一种这样的淋巴因子，并通过凝胶过滤鉴定为一种表观分子量为 40,000 至 50,000 的物质，类似于 BCGF II。它支持 B 细胞母细胞的增殖并诱导它们分化为形成噬斑细胞。诸如 BCGF I、白细胞介素 2 和 BCDFγ 等淋巴因子既不能维持山羊抗 IgM 预激活的 B 淋巴细胞的生长，也不能诱导其分化。
