Research Unit of Integrated Crop Production, Centre Regional de la Recherche Agronomique d'Agadir, Agadir, Morocco.
Biotechnology and Environmental Engineering Team, Laboratory of Mechanic Process Energy and Environment, National School of Applied Sciences, Ibn Zohr University, Agadir, Morocco.
Sci Rep. 2019 Sep 6;9(1):12832. doi: 10.1038/s41598-019-49216-8.
Pseudomonas isolates have frequently been isolated from the rhizosphere of plants, and several of them have been reported as plant growth-promoting rhizobacteria. In the present work, tomato (Solanum lycopersicum) seeds were germinated in greenhouse conditions, and the seedling height, length of plants, collar diameter and number of leaves were measured from plants grown in soil inoculated by bacterial isolates. Pseudomonas isolates were isolated from the rhizosphere. We used the Newman-Keuls test to ascertain pairwise differences. Isolates were identified as a new Pseudomonas species by rpoD gene sequencing. The results showed that isolates of Pseudomonas sp. (Q6B) increased seed germination (P = 0.01); Pseudomonas sp. (Q6B, Q14B, Q7B, Q1B and Q13B) also promoted seedling height (P = 0.01). All five isolates promoted plant length and enlarged the collar diameter (P = 0.01). Pseudomonas sp. (Q1B) also increased leaf number (P = 0.01). The investigation found that Pseudomonas isolates were able to solubilize phosphate, produce siderophores, ammonia, and indole-3-acetic acid and colonize the roots of tomato plants. This study shows that these five novel Pseudomonas sp. isolates can be effective new plant growth-promoting rhizobacteria.
从植物根际中经常分离到假单胞菌分离株，其中一些已被报道为植物促生根际细菌。在本工作中，番茄（Solanum lycopersicum）种子在温室条件下发芽，从接种细菌分离株的土壤中生长的植物中测量幼苗高度、植物长度、颈直径和叶片数。从根际中分离出假单胞菌分离株。我们使用 Newman-Keuls 检验来确定成对差异。通过 rpoD 基因测序将分离株鉴定为新的假单胞菌种。结果表明，假单胞菌属（Q6B）分离株增加了种子发芽（P=0.01）；假单胞菌属（Q6B、Q14B、Q7B、Q1B 和 Q13B）也促进了幼苗高度（P=0.01）。所有五个分离株都促进了植物长度的增加，并扩大了颈直径（P=0.01）。假单胞菌属（Q1B）还增加了叶片数（P=0.01）。研究发现，假单胞菌分离株能够溶解磷酸盐、产生铁载体、氨、吲哚-3-乙酸并定殖番茄植物的根。本研究表明，这五个新的假单胞菌属分离株可以成为有效的新型植物促生根际细菌。
