Ali Md Sahadat, Mony Fatima Tuz Zohora, Evans Michael, Rideout Steven, Haak David, Vieira Paulo, Eisenback Jonathan D
School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, Virginia, United States of America.
Mycology and Nematology Genetic Diversity and Biology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, United States of America.
PLoS One. 2025 Sep 11;20(9):e0331920. doi: 10.1371/journal.pone.0331920. eCollection 2025.
A Gram-negative, facultative anaerobic, rod-shaped, motile with peritrichous flagella, fluorescent bacterium, designated 'Candidatus Pseudomonas auctus' sp. nov. JDE115, was isolated from soybean root nodules in Virginia and characterized using a comprehensive integrative methodology. Growth of JDE115 occurred with 0-5.0% (w/v) NaCl (optimum 1%), at pH 6.0-10.0 (optimum pH 7.0), and at 10-40°C (optimum 28°C) in LB broth. Phylogenetic analyses based on the 16S rRNA gene placed the isolate as a member of a novel species within the genus Pseudomonas. Phylogenetic analyses based on whole-genome sequences, 16S rRNA, showed JDE115 having the highest similarity to Pseudomonas glycinae MS586. Average Nucleotide Identity (ANI) analysis also revealed the highest similarity of JDE115 to Pseudomonas glycinae MS586 (94.59%), which is below the 95% threshold for species delineation. Genome-to-genome distance analysis (GGDC, Formula 2) showed a maximum value of 57.10% with the same strain, far below the 70% cutoff. The primary isoprenoid quinone detected in JDE115 was ubiquinone-9 (Q-9) and the DNA G + C content was 60.68 mol%. The whole-cell fatty acid profile was dominated by C16:0, C17:0 cyclo, and the summed features 3 (C16:1ω7c and/or C16:1ω6c) and 8 (C18:1ω7c and/or C18:1ω6c). Additional fatty acids detected included 12:0, 14:0, and 18:0. Based on these phenotypic, chemotaxonomic, and phylogenetic data, strain JDE115 is proposed to represent a new species in the genus Pseudomonas, for which the name 'Candidatus Pseudomonas auctus' sp. nov. is proposed.
从弗吉尼亚州大豆根瘤中分离出一株革兰氏阴性、兼性厌氧、杆状、具周生鞭毛能运动的荧光细菌，命名为“新种暂定假单胞菌（Candidatus Pseudomonas auctus）”JDE115，并采用综合方法对其进行了表征。JDE115在LB肉汤中，NaCl浓度为0 - 5.0%（w/v）（最适浓度1%）、pH值为6.0 - 10.0（最适pH值7.0）、温度为10 - 40°C（最适温度28°C）条件下生长。基于16S rRNA基因的系统发育分析将该分离株置于假单胞菌属内一个新物种的成员之中。基于全基因组序列的16S rRNA系统发育分析表明，JDE115与大豆假单胞菌MS586的相似性最高。平均核苷酸同一性（ANI）分析也显示JDE115与大豆假单胞菌MS586的相似性最高（94.59%），低于物种划分的95%阈值。基因组到基因组距离分析（GGDC，公式2）显示与同一菌株的最大值为57.10%，远低于70%的临界值。在JDE115中检测到的主要类异戊二烯醌为泛醌-9（Q-9），DNA的G + C含量为60.68 mol%。全细胞脂肪酸谱以C16:0、C17:0环丙烷以及总和特征3（C16:1ω7c和/或C16:1ω6c）和8（C18:1ω7c和/或C18:1ω6c）为主。检测到的其他脂肪酸包括12:0、14:0和18:0。基于这些表型、化学分类和系统发育数据，菌株JDE115被提议代表假单胞菌属中的一个新物种，为此提议命名为“新种暂定假单胞菌（Candidatus Pseudomonas auctus）”。
