School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji'nan 250117, PR China.
Key Laboratory of Emerging Infectious Diseases in Universities of Shandong, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian 271000, PR China.
J Gen Virol. 2024 May;105(5). doi: 10.1099/jgv.0.001989.
Hepeviruses have been identified in a broad range of animal hosts, including mammals, birds, and fish. In this study, rodents (=91) from seven different species and ten pikas () were collected in Qinghai Province, China. Using transcriptomic sequencing and confirmatory molecular testing, hepeviruses were detected in 27 of 45 (60 %) long-tailed dwarf hamsters () and were undetected in other rodents and pika. The complete genome sequences from 14 representative strains were subsequently obtained, and phylogenetic analyses suggested that they represent a novel species within the genus , which we tentatively designated as Cl-2018QH. The virus was successfully isolated in human hepatoma (Huh-7) and murine fibroblast (17 Cl-1) cell lines, though both exhibited limited replication as assayed by detection of negative-sense RNA intermediates. A129 immunodeficient mice were inoculated with Cl-2018QH and the virus was consistently detected in multiple organs, despite relatively low viral loads. In summary, this study has described a novel rodent hepevirus, which enhances our knowledge of the genetic diversity of rodent hepeviruses and highlights its potential for cross-species transmission.
已在包括哺乳动物、鸟类和鱼类在内的广泛动物宿主中鉴定出了 Hepeviruses。在这项研究中，从中国青海省收集了七种不同物种的啮齿动物（=91 只）和十只鼠兔。使用转录组测序和确证性分子检测，在 45 只（60%）长尾侏儒仓鼠（）中检测到了 Hepeviruses，而在其他啮齿动物和鼠兔中未检测到。随后获得了 14 个代表性菌株的完整基因组序列，系统发育分析表明它们代表了属中的一个新种，我们暂定将其命名为 Cl-2018QH。该病毒在人肝癌（Huh-7）和鼠成纤维细胞（17 Cl-1）细胞系中成功分离，但如通过检测负义 RNA 中间体所测定的，两种细胞系的复制均有限。A129 免疫缺陷小鼠接种 Cl-2018QH 后，病毒在多个器官中持续检测到，尽管病毒载量相对较低。总之，这项研究描述了一种新型啮齿动物 Hepevirus，这增强了我们对啮齿动物 Hepeviruses 遗传多样性的认识，并突出了其跨物种传播的潜力。
