Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
Henan University of Chinese Medicine, Zhengzhou, Henan 450046, PR China.
Fitoterapia. 2024 Jul;176:106030. doi: 10.1016/j.fitote.2024.106030. Epub 2024 May 19.
Four pairs of undescribed enantiomeric guaiane sesquiterpenoids, (±)-alismaenols A-D (1a/1b, 3a/3b-5a/5b), together with a pair of known ones (2a/2b) were isolated from the rhizomes of Alisma plantago-aquatica. The structures and relative configurations of the isolates were established by analysis of their 1D, 2D-NMR and HRESIMS data. Their absolute configurations were determined by comparison of their experimental CD spectra and calculated electronic circular dichroism (ECD) spectra or by single-crystal X-ray diffraction analysis. All compounds (1a/1b-5a/5b) were evaluated for their inhibitory effects on nitric oxide (NO) production in LPS-induced RAW 264.7 cells, and compound 1a exhibited stronger activity (IC = 12.89 μM) than indomethacin (IC = 14.03 μM).
从泽泻的根茎中分离得到了四对未描述的对映异构的愈创木烷倍半萜 (±)-alismaenols A-D (1a/1b, 3a/3b-5a/5b)，以及一对已知的 (2a/2b)。通过分析它们的 1D、2D-NMR 和 HRESIMS 数据确定了这些分离物的结构和相对构型。通过比较它们的实验 CD 光谱和计算的电子圆二色性 (ECD) 光谱或通过单晶 X 射线衍射分析确定了它们的绝对构型。所有化合物 (1a/1b-5a/5b) 都评估了它们对 LPS 诱导的 RAW 264.7 细胞中一氧化氮 (NO) 产生的抑制作用，化合物 1a 表现出比吲哚美辛更强的活性 (IC = 12.89 μM)。
