Department of Biochemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.
Department of Chemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.
Sci Rep. 2024 May 20;14(1):11450. doi: 10.1038/s41598-024-62409-0.
A mesoporous silica nanoparticle (MSN) coated with polydopamine (PDA) and loaded with umbelliprenin (UMB) was prepared and evaluated for its anti-cancer properties in this study. Then UMB-MSN-PDA was characterized by dynamic light scattering (DLS), Field emission scanning electron microscopy (FESEM), Transmission electron microscopy (TEM) and FTIR methods. UV-visible spectrometry was employed to study the percentage of encapsulation efficiency (EE%). UMB-MSN-PDA mediated cell cytotoxicity and their ability to induce programmed cell death were evaluated by MTT, real-time qPCR, flow cytometry, and AO/PI double staining methods. The size of UMB-MSN-PDA was 196.7 with a size distribution of 0.21 and a surface charge of -41.07 mV. The EE% was 91.92%. FESEM and TEM showed the spherical morphology of the UMB-MSN-PDA. FTIR also indicated the successful interaction of the UMB and MSN and PDA coating. The release study showed an initial 20% release during the first 24 h of the study and less than 40% during 168 h. The lower cytotoxicity of the UMB-MSN-PDA against HFF normal cells compared to MCF-7 carcinoma cells suggested the safety of formulation on normal cells and tissues. The induction of apoptosis in MCF-7 cells was indicated by the upregulation of P53, caspase 8, and caspase 9 genes, enhanced Sub-G1 phase cells, and the AO/PI fluorescent staining. As a result of these studies, it may be feasible to conduct preclinical studies shortly to evaluate the formulation for its potential use in cancer treatment.
本研究制备了一种载有 Umbelliprenin（UMB）的介孔硅纳米粒子（MSN），并用聚多巴胺（PDA）进行了涂层，并对其抗癌性能进行了评价。然后通过动态光散射（DLS）、场发射扫描电子显微镜（FESEM）、透射电子显微镜（TEM）和傅里叶变换红外光谱（FTIR）方法对 UMB-MSN-PDA 进行了表征。采用紫外可见分光光度法研究包封效率（EE%）。通过 MTT、实时 qPCR、流式细胞术和 AO/PI 双重染色法评估了 UMB-MSN-PDA 介导的细胞细胞毒性及其诱导程序性细胞死亡的能力。UMB-MSN-PDA 的粒径为 196.7nm，粒径分布为 0.21，表面电荷为-41.07mV。EE%为 91.92%。FESEM 和 TEM 显示 UMB-MSN-PDA 呈球形形态。FTIR 还表明 UMB 与 MSN 和 PDA 涂层的成功相互作用。释放研究表明，在研究的前 24 小时内，有 20%的药物初始释放，而在 168 小时内，释放量小于 40%。与 MCF-7 癌细胞相比，UMB-MSN-PDA 对 HFF 正常细胞的较低细胞毒性表明该制剂对正常细胞和组织的安全性。MCF-7 细胞中 P53、caspase 8 和 caspase 9 基因的上调、Sub-G1 期细胞的增强以及 AO/PI 荧光染色表明细胞凋亡的诱导。基于这些研究，可能很快就可以进行临床前研究，以评估该制剂在癌症治疗中的潜在用途。
