The Affiliated Stomatological Hospital, Jiangxi Medical College, Nanchang University & Jiangxi Province Key Laboratory of Oral Biomedicine & Jiangxi Province Clinical Research Center for Oral Diseases, Nanchang, Jiangxi, China.
Proteomics Clin Appl. 2024 Nov;18(6):e2400006. doi: 10.1002/prca.202400006. Epub 2024 May 20.
To screen differentially expressed proteins (DEPs) in the saliva of Early childhood caries (ECC) with different degrees of severity.
The proteomic profiles of salivary of children with ECC of varying severity by data independent acquisition data independent acquisition (DIA) technique. A total of 12 preschool children aged 3-5 years were included in this study.
In this study, a total of 15,083 peptides and 1944 proteins were quantified; The results of DEPs screening showed that 34 DEPs were identified between the group H and the group LC, including 18 up-regulated proteins and 16 down-regulated proteins; 34 DEPs were screened between the group H and the group HC, including 17 up-regulated proteins and 17 down-regulated proteins; 42 DEPs were screened between the group LC and the group HC, including 18 up-regulated proteins and 24 down-regulated proteins. Among these DEPs, we screened several key proteins that may play a role in ECC, such as MK, histone H4, TGFβ3, ZG16B, MUC20, and SMR-3B.
Salivary proteins, as important host factors of caries, are differentially expressed between the saliva of ECC children and healthy children. Specific DEPs are expected to become potential biomarkers for the diagnosis of ECC.
筛选不同严重程度婴幼儿龋（ECC）患者唾液中的差异表达蛋白（DEPs）。
采用数据非依赖性采集（DIA）技术对不同严重程度 ECC 患儿唾液中的蛋白质组谱进行分析。
本研究共定量了 15083 个肽段和 1944 个蛋白质；DEPs 筛选结果显示，H 组与 LC 组之间有 34 个 DEPs，包括 18 个上调蛋白和 16 个下调蛋白；H 组与 HC 组之间有 34 个 DEPs，包括 17 个上调蛋白和 17 个下调蛋白；LC 组与 HC 组之间有 42 个 DEPs，包括 18 个上调蛋白和 24 个下调蛋白。在这些 DEPs 中，我们筛选出了几个可能在 ECC 中发挥作用的关键蛋白，如 MK、组蛋白 H4、TGFβ3、ZG16B、MUC20 和 SMR-3B。
唾液蛋白作为龋病的重要宿主因素，在 ECC 患儿和健康儿童的唾液中存在差异表达。特定的 DEPs 有望成为 ECC 诊断的潜在生物标志物。
