Ahmad Paras, Hussain Ahmed, Siqueira Walter L
College of Dentistry, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Mass Spectrom Rev. 2024 Jul-Aug;43(4):826-856. doi: 10.1002/mas.21822. Epub 2022 Nov 29.
Dental caries is a multifactorial chronic disease resulting from the intricate interplay among acid-generating bacteria, fermentable carbohydrates, and several host factors such as saliva. Saliva comprises several proteins which could be utilized as biomarkers for caries prevention, diagnosis, and prognosis. Mass spectrometry-based salivary proteomics approaches, owing to their sensitivity, provide the opportunity to investigate and unveil crucial cariogenic pathogen activity and host indicators and may demonstrate clinically relevant biomarkers to improve caries diagnosis and management. The present review outlines the published literature of human clinical proteomics investigations on caries and extensively elucidates frequently reported salivary proteins as biomarkers. This review also discusses important aspects while designing an experimental proteomics workflow. The protein-protein interactions and the clinical relevance of salivary proteins as biomarkers for caries, together with uninvestigated domains of the discipline are also discussed critically.
龋齿是一种多因素慢性疾病，由产酸细菌、可发酵碳水化合物以及唾液等多种宿主因素之间复杂的相互作用导致。唾液包含多种蛋白质，这些蛋白质可用作龋齿预防、诊断和预后的生物标志物。基于质谱的唾液蛋白质组学方法因其灵敏度高，为研究和揭示关键致龋病原体活性及宿主指标提供了机会，并且可能展示出具有临床相关性的生物标志物以改善龋齿的诊断和管理。本综述概述了已发表的关于龋齿的人类临床蛋白质组学研究文献，并广泛阐明了经常报道的作为生物标志物的唾液蛋白质。本综述还讨论了设计实验性蛋白质组学工作流程时的重要方面。还对唾液蛋白质作为龋齿生物标志物的蛋白质-蛋白质相互作用及其临床相关性，以及该学科未研究的领域进行了批判性讨论。
