Ding Hui, Chen Xiao-Chun, Wan Lin, Zhang Ying-Ying, Rui Xiao-Hong, He Tian, Liu Jun, Shang Zhong-Bo
Department of Laboratory Medicine, Affiliated Wuxi Fifth Hospital of Jiangnan University, Wuxi, 214005, China.
Department of Laboratory Medicine, Taizhou Second People's Hospital, Taizhou, 225411, China.
Heliyon. 2024 May 7;10(10):e30829. doi: 10.1016/j.heliyon.2024.e30829. eCollection 2024 May 30.
is an opportunistic pathogen causing severe infections. The circadian rhythm is the internal rhythm mechanism of an organism and plays an important role in coping with changes in the 24-h circadian rhythm. Disruption of the circadian rhythm can lead to immune, behavioral, mental, and other related disorders. Whether can disrupt the circadian rhythm after infection remains unclear. Here, we examined the effects of NTUH-K2044 infection on biological rhythm and inflammation in zebrafish using behavioral assays, quantitative real-time reverse transcription PCR, neutrophil and macrophage transgenic fish, and drug treatment. The results showed that infection decreased the motor activity of zebrafish and reduced the circadian rhythm amplitude, phase, and period. The expression of core circadian rhythm-associated genes increased under light-dark conditions, whereas they were downregulated under continuous darkness. Analysis of mediated inflammation using Tg(mpx:EGFP) and Tg(mpeg:EGFP) transgenic zebrafish, expressing fluorescent neutrophils and macrophages, respectively, showed increased induction of inflammatory cells, upregulated expression of inflammatory factor genes, and stronger inflammatory responses under light-dark conditions. These effects were reversed by the anti-inflammatory drug G6PDi-1, and the expression of clock genes following treatment was disrupted. We determined the relationship among , inflammation, and the circadian rhythm, providing a theoretical reference for studying circadian rhythm disorders caused by inflammation.
是一种引起严重感染的机会致病菌。昼夜节律是生物体的内部节律机制，在应对24小时昼夜节律变化中起重要作用。昼夜节律的破坏可导致免疫、行为、心理和其他相关紊乱。感染后是否会破坏昼夜节律仍不清楚。在这里，我们使用行为分析、定量实时逆转录PCR、中性粒细胞和巨噬细胞转基因鱼以及药物治疗，研究了NTUH-K2044感染对斑马鱼生物节律和炎症的影响。结果表明，感染降低了斑马鱼的运动活性，降低了昼夜节律的幅度、相位和周期。核心昼夜节律相关基因的表达在明暗条件下增加，而在持续黑暗条件下则下调。分别使用表达荧光中性粒细胞和巨噬细胞的Tg(mpx:EGFP)和Tg(mpeg:EGFP)转基因斑马鱼对介导的炎症进行分析，结果显示在明暗条件下炎症细胞的诱导增加、炎症因子基因的表达上调以及更强的炎症反应。这些作用被抗炎药物G6PDi-1逆转，并且治疗后生物钟基因的表达被破坏。我们确定了、炎症和昼夜节律之间的关系，为研究由炎症引起的昼夜节律紊乱提供了理论参考。 （注：原文中部分“ ”指代不明，翻译可能存在一定局限性）
