Singer C J, Katz S E
J Assoc Off Anal Chem. 1985 Sep-Oct;68(5):1037-41.
Procedures for the assay of chloramphenicol in milk, urine, serum, and muscle tissue are presented. The procedures specify an assay design with all standards as well as samples present on each plate, oxytetracycline in the buffer-diluent for greater sensitivity, a minimal medium to enhance the inhibitory effect of chloramphenicol on the assay organism, and a tetrazolium dye to improve the ability to measure the zones of inhibition. Recoveries of unbound chloramphenicol from bovine urine were 90.8%, from serum 88.3%, from milk 79.3%, from swine muscle 71.3%, and from beef and chicken muscle 61.0 and 61.4%, respectively. The lower levels of measurement in urine and serum were 0.25 microgram/mL, 0.025 microgram/mL in milk, and 0.10 microgram/g in muscle tissue.
介绍了牛奶、尿液、血清和肌肉组织中氯霉素的检测方法。这些方法规定了一种检测设计，即每个平板上都有所有标准品以及样品，缓冲稀释液中含有土霉素以提高灵敏度，使用一种基本培养基来增强氯霉素对检测生物的抑制作用，以及使用一种四氮唑染料来提高测量抑菌圈的能力。未结合氯霉素从牛尿液中的回收率为90.8%，从血清中为88.3%，从牛奶中为79.3%，从猪肌肉中为71.3%，从牛肉和鸡肉中分别为61.0%和61.4%。尿液和血清中的最低检测水平分别为0.25微克/毫升、牛奶中为0.025微克/毫升、肌肉组织中为0.10微克/克。
