Biassoni R, Prigione I, Di Marco E, Ferrini S
Ric Clin Lab. 1985 Apr-Jun;15(2):177-83. doi: 10.1007/BF03029837.
T lymphocytes were isolated from ascitic fluid of three patients with ovarian carcinoma at III-IV stage. Surface markers analysis of such purified T cells revealed that T8+ cells were well represented among ascitic T lymphocytes (from 35 to 56%). Low percentages of activated T cells, as indicated by HLA-DR and TAC (interleukin-2 receptor) positivity, were also present. However, fresh ascitic T lymphocytes failed to lyse autologous tumor target cells in a 4-h 51Cr release assay. Furthermore, by applying a limiting dilution microculture system that allows optimal conditions for cloning of human T lymphocytes, we derived clones from these populations. From 41 to 63% of clones so obtained had cytolytic activity in a lectin-dependent assay allowing detection of cytolytic T cells of any specificity. More importantly, in all three patients several clones were found to lyse autologous tumor target cells as well. Some of these clones have been studied in more detail: 9 out of 10 expressed the T8+/T4- phenotype, whereas only one was T8-/T4+; 6 out of 9 clones had a definite NK-like activity, while none of them lysed autologous PHA-lymphoblasts.
从三名处于III - IV期的卵巢癌患者的腹水中分离出T淋巴细胞。对这些纯化的T细胞进行表面标志物分析发现，腹水中的T淋巴细胞中T8 +细胞占比很高（35%至56%）。同时也存在低比例的活化T细胞，这可通过HLA - DR和TAC（白细胞介素 - 2受体）阳性来表明。然而，在4小时的51Cr释放试验中，新鲜的腹水T淋巴细胞未能裂解自体肿瘤靶细胞。此外，通过应用一种能为人T淋巴细胞克隆提供最佳条件的有限稀释微培养系统，我们从这些细胞群体中获得了克隆。在依赖凝集素的试验中，如此获得的41%至63%的克隆具有细胞溶解活性，该试验可检测任何特异性的细胞溶解T细胞。更重要的是，在所有三名患者中，还发现有几个克隆也能裂解自体肿瘤靶细胞。其中一些克隆已得到更详细的研究：10个中有9个表现出T8 + / T4 - 表型，而只有1个是T8 - / T4 +；9个克隆中有6个具有明确的NK样活性，而它们均未裂解自体PHA淋巴细胞母细胞。
