Ferrini S, Biassoni R, Moretta A, Bruzzone M, Nicolin A, Moretta L
Int J Cancer. 1985 Sep 15;36(3):337-43.
T lymphocytes isolated from ascitic fluid of patients with stage III-IV ovarian carcinoma were cloned by means of a microculture system that allows cloning of virtually all peripheral blood human T lymphocytes. Under these experimental conditions, 15% to 42% of ascitic T cells gave rise to clonal progenies that were analyzed for different functional capabilities. Of the clones obtained, 36%-70% had cytolytic activity in a PHA-dependent assay (using P815 as target cells) that allowed detection of cytolytic cells of any specificity. About one-half of the cytolytic clones lysed the NK-sensitive K562 target cells as well. In addition, 30%-50% of the total clones released IL-2 upon stimulation with PHA for 24 hr. In all patients analyzed a variable proportion (11%-56% of all cytolytic clones) had cytolytic activity against autologous tumor cells. Some of these clones have been analyzed in more detail: 18/21 expressed the T4-T8+ phenotype, whereas the remaining 3 were T4+ T8-. Only one out of 6 clones tested lysed allogeneic ovarian carcinoma cells as well, while 5/8 had a definite NK-like activity. Finally, all 8 clones tested were inhibited by anti-T11 and 7/8 by anti-T8 monoclonal antibody.
从 III - IV 期卵巢癌患者腹水中分离出的 T 淋巴细胞，通过一种微培养系统进行克隆，该系统几乎能克隆所有外周血人类 T 淋巴细胞。在这些实验条件下，15%至 42%的腹水 T 细胞产生了克隆后代，并对其不同功能能力进行了分析。在所获得的克隆中，36% - 70%在依赖 PHA 的检测中（使用 P815 作为靶细胞）具有细胞溶解活性，该检测能检测到任何特异性的细胞溶解细胞。约一半的细胞溶解克隆也能裂解 NK 敏感的 K562 靶细胞。此外，在用 PHA 刺激 24 小时后，30% - 50%的总克隆释放白细胞介素 - 2。在所有分析的患者中，可变比例（占所有细胞溶解克隆的 11% - 56%）对自体肿瘤细胞具有细胞溶解活性。其中一些克隆已进行了更详细的分析：21 个中有 18 个表达 T4 - T8 + 表型，而其余 3 个为 T4 + T8 - 。在测试的 6 个克隆中，只有 1 个也能裂解同种异体卵巢癌细胞，而 8 个中有 5 个具有明确的 NK 样活性。最后，测试的所有 8 个克隆均被抗 T11 抑制，8 个中有 7 个被抗 T8 单克隆抗体抑制。
