Vivino A A, Boraker D K, Miller D, Nyborg W
Ultrasound Med Biol. 1985 Sep-Oct;11(5):751-9. doi: 10.1016/0301-5629(85)90109-7.
Murine spleen cell suspensions stimulated by Concanavalin A (Con-A) were exposed to 1.6-MHz continuous-wave ultrasound at low intensities (spatial-peak values ranging from 16 to 300 mW/cm2) in the presence of a Nuclepore membrane that contained stabilized gas bodies. The ultrasonically activated gas bodies induced cell lysis and reduced the fraction of intact cells that excluded trypan blue. At a spatial-peak intensity of 75 mW/cm2 (spatial-average intensity 15 mW/cm2), Con-A-induced Methyl[3H]thymidine (3H-TdR) incorporation was reduced in cultures exposed at 24 or 48 hr after addition of Con-A but not at 7 or 13 hr. There was no observable effect on cell survival or 3H-TdR incorporation at spatial-peak intensities below 75 mW/cm2.
用刀豆球蛋白A（Con-A）刺激的小鼠脾细胞悬液，在含有稳定气体小体的核孔膜存在的情况下，暴露于低强度（空间峰值范围为16至300 mW/cm²）的1.6 MHz连续波超声下。超声激活的气体小体诱导细胞裂解，并减少了排斥台盼蓝的完整细胞比例。在空间峰值强度为75 mW/cm²（空间平均强度15 mW/cm²）时，在添加Con-A后24或48小时暴露的培养物中，Con-A诱导的甲基[³H]胸腺嘧啶核苷（³H-TdR）掺入减少，但在7或13小时时未减少。在空间峰值强度低于75 mW/cm²时，对细胞存活或³H-TdR掺入没有可观察到的影响。
