白色念珠菌几丁质合成酶的调节与增溶作用
Regulation and solubilization of Candida albicans chitin synthetase.
作者信息
Braun P C, Calderone R A
出版信息
J Bacteriol. 1979 Nov;140(2):666-70. doi: 10.1128/jb.140.2.666-670.1979.
Abstract
A cytoplasmic component which inhibited the activation of chitin synthetase was studied in the dimorphic fungus Candida albicans. The inhibitor was found to be heat stable and trypsin sensitive and was only effective when incubated with a vacuolar protease, an activator of chitin synthetase, before the activation of chitin synthetase. In addition, the particulate chitin synthetase from the yeast form of C. albicans was solubilized by a sodium cholate-digitonin extraction and subsequently was purified approximately 30-fold by Sepharose column chromatography and Amicon XM 100 filtration. Activity of the soluble enzyme was increased by the addition of trypsin or phosphatidyl serine. The molecular weight of the enzyme was estimated to be 400,000.
摘要
在二态真菌白色念珠菌中研究了一种抑制几丁质合成酶激活的细胞质成分。发现该抑制剂对热稳定且对胰蛋白酶敏感，并且仅在几丁质合成酶激活之前与液泡蛋白酶（一种几丁质合成酶激活剂）一起孵育时才有效。此外，白色念珠菌酵母形式的颗粒状几丁质合成酶通过胆酸钠-洋地黄皂苷提取物溶解，随后通过琼脂糖柱色谱和Amicon XM 100过滤纯化约30倍。通过添加胰蛋白酶或磷脂酰丝氨酸可增加可溶性酶的活性。该酶的分子量估计为400,000。
相似文献
1
Regulation and solubilization of Candida albicans chitin synthetase.白色念珠菌几丁质合成酶的调节与增溶作用
J Bacteriol. 1979 Nov;140(2):666-70. doi: 10.1128/jb.140.2.666-670.1979.
2
Chitin synthase in Candida albicans: comparison of digitonin-permeabilized cells and spheroplast membranes.白色念珠菌中的几丁质合成酶：洋地黄皂苷透化细胞与原生质体膜的比较
J Bacteriol. 1985 May;162(2):826-9. doi: 10.1128/jb.162.2.826-829.1985.
3
Solubilization and partial purification of yeast chitin synthetase. Confirmation of the zymogenic nature of the enzyme.酵母几丁质合成酶的增溶与部分纯化。该酶酶原性质的确认。
J Biol Chem. 1978 Jun 25;253(12):4419-25.
4
Effect of digitonin on membrane-bound and chitosomal chitin synthetase activity in protoplasts from yeast cells of Candida albicans.洋地黄皂苷对白色念珠菌酵母细胞原生质体中膜结合型和几丁质体几丁质合成酶活性的影响。
Antonie Van Leeuwenhoek. 1993;64(1):67-74. doi: 10.1007/BF00870923.
5
Chitin synthesis in Candida albicans: comparison of yeast and hyphal forms.白色念珠菌中的几丁质合成：酵母形态与菌丝形态的比较。
J Bacteriol. 1978 Mar;133(3):1472-7. doi: 10.1128/jb.133.3.1472-1477.1978.
6
Activation of chitin synthetase in permeabilized cells of a Saccharomyces cerevisiae mutant lacking proteinase B.在缺乏蛋白酶B的酿酒酵母突变体的透化细胞中几丁质合成酶的激活。
J Bacteriol. 1982 Dec;152(3):1255-64. doi: 10.1128/jb.152.3.1255-1264.1982.
7
Regulation of chitin synthesis during germ-tube formation in Candida albicans.白色念珠菌芽管形成过程中几丁质合成的调控。
Arch Microbiol. 1980 Mar;125(1-2):97-104. doi: 10.1007/BF00403204.
8
Isolation of chitin synthetase from Saccharomyces cerevisiae. Purification of an enzyme by entrapment in the reaction product.从酿酒酵母中分离几丁质合成酶。通过截留于反应产物中来纯化一种酶。
J Biol Chem. 1984 Dec 10;259(23):14966-72.
9
The effects of ergosterol and alcohols on germ-tube formation and chitin synthase in Candida albicans.
Can J Biochem. 1982 Jan;60(1):15-20. doi: 10.1139/o82-003.
10
Properties of chitin synthetase in isolated chitosomes from yeast cells of Mucor rouxii.来自鲁氏毛霉酵母细胞的分离几丁质体中几丁质合成酶的特性
J Biol Chem. 1977 May 25;252(10):3338-43.
引用本文的文献
1
Effect of digitonin on membrane-bound and chitosomal chitin synthetase activity in protoplasts from yeast cells of Candida albicans.洋地黄皂苷对白色念珠菌酵母细胞原生质体中膜结合型和几丁质体几丁质合成酶活性的影响。
Antonie Van Leeuwenhoek. 1993;64(1):67-74. doi: 10.1007/BF00870923.
2
Comparative biochemistry of the proteinases of eucaryotic microorganisms.真核微生物蛋白酶的比较生物化学
Microbiol Rev. 1982 Sep;46(3):308-40. doi: 10.1128/mr.46.3.308-340.1982.
3
Chitin synthetase from the yeast and mycelial phases of Blastomyces dermatitidis.皮炎芽生菌酵母期和菌丝期的几丁质合成酶
Mycopathologia. 1985 May;90(2):91-6. doi: 10.1007/BF00436856.
4
Chitin synthase in Candida albicans: comparison of digitonin-permeabilized cells and spheroplast membranes.白色念珠菌中的几丁质合成酶：洋地黄皂苷透化细胞与原生质体膜的比较
J Bacteriol. 1985 May;162(2):826-9. doi: 10.1128/jb.162.2.826-829.1985.
5
Chitin synthase in encysting Entamoeba invadens.侵袭内阿米巴包囊化过程中的几丁质合酶
Biochem J. 1991 Dec 15;280 ( Pt 3)(Pt 3):641-7. doi: 10.1042/bj2800641.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Chitin and yeast budding. Allosteric inhibition of chitin synthetase by a heat-stable protein from yeast.几丁质与酵母出芽。酵母中一种热稳定蛋白对几丁质合成酶的变构抑制作用。
J Biol Chem. 1971 Jan 10;246(1):167-73.
3
The control of morphogenesis: an enzymatic mechanism for the initiation of septum formation in yeast.形态发生的控制：酵母中隔膜形成起始的一种酶促机制。
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2052-6. doi: 10.1073/pnas.68.9.2052.
4
Inhibition and activation of chitin synthesis by Mucor rouxii cell extracts.鲁氏毛霉细胞提取物对几丁质合成的抑制与激活作用。
Arch Biochem Biophys. 1973 Oct;158(2):812-6. doi: 10.1016/0003-9861(73)90576-6.
5
Yeast glycogen synthetase in the glucose 6-phosphate independent form: a case of cold lability without major changes in molecular size.
Biochim Biophys Acta. 1973 Apr 12;302(2):240-8. doi: 10.1016/0005-2744(73)90153-8.
6
7
Molecular aspects of yeast morphogenesis.酵母形态发生的分子层面
Annu Rev Microbiol. 1975;29:191-214. doi: 10.1146/annurev.mi.29.100175.001203.
8
Solubilization and partial purification of yeast chitin synthetase. Confirmation of the zymogenic nature of the enzyme.酵母几丁质合成酶的增溶与部分纯化。该酶酶原性质的确认。
J Biol Chem. 1978 Jun 25;253(12):4419-25.
9
Chitin synthesis in Candida albicans: comparison of yeast and hyphal forms.白色念珠菌中的几丁质合成：酵母形态与菌丝形态的比较。
J Bacteriol. 1978 Mar;133(3):1472-7. doi: 10.1128/jb.133.3.1472-1477.1978.
Zotero 插件