Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Department of Medical Microbiology & Immunology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Wound Repair Regen. 2024 Sep-Oct;32(5):675-685. doi: 10.1111/wrr.13190. Epub 2024 May 22.
Porcine models are frequently used for burn healing studies; however, factors including anatomic location and lack of standardised wound methods can impact the interpretation of wound data. The objectives of this study are to examine the influence of anatomical locations on the uniformity of burn creation and healing in porcine burn models. To optimise burn parameters on dorsal and ventral surfaces, ex vivo and in situ euthanized animals were first used to examine the location-dependence of the burn depth and contact time relationship. The location-dependent healing in vivo was then examined using burn and excisional wounds at dorsal, ventral, caudal and cranial locations. Lactate dehydrogenase (LDH) and H&E were used to assess burn depth and wound re-epithelialization. We found that burn depth on the ventral skin was significantly deeper than that of the dorsal skin at identical thermal conditions. Compared with burns created ex vivo, burns created in situ immediately post-mortem were significantly deeper in the ventral location. In live animals, 2 out of 12 burn wounds were fully re-epithelialized after 14 days in contrast to complete re-epithelialization of all excisional wounds. Among the burn wounds, those at the cranial-dorsal site exhibited faster healing than at the caudal-dorsal site. This study showed that anatomical location is an important consideration for the consistency of burn depth creation and healing. These data support symmetric localization of treatment and control for comparative assessment of burn healing in porcine models to prevent misinterpretation of results and increase the translatability of findings to humans.
猪模型常用于烧伤愈合研究；然而，包括解剖部位和缺乏标准化伤口方法在内的多种因素会影响伤口数据的解读。本研究旨在检验解剖部位对猪烧伤模型中烧伤形成和愈合均匀性的影响。为了优化背侧和腹侧表面的烧伤参数，首先使用离体和原位安乐死动物来检验烧伤深度和接触时间关系的位置依赖性。然后，在背侧、腹侧、尾侧和颅侧位置分别进行烧伤和切除伤口，以检验体内位置依赖性愈合。使用乳酸脱氢酶 (LDH) 和 H&E 评估烧伤深度和伤口再上皮化。我们发现，在相同的热条件下，腹侧皮肤的烧伤深度明显比背侧皮肤深。与离体烧伤相比，在死后即刻原位烧伤的腹侧位置明显更深。在活体动物中，12 个烧伤伤口中有 2 个在 14 天后完全再上皮化，而所有切除伤口均完全再上皮化。在烧伤伤口中，颅侧-背侧部位的愈合速度快于尾侧-背侧部位。本研究表明，解剖部位是影响烧伤深度形成和愈合一致性的重要因素。这些数据支持在猪模型中对烧伤愈合进行对称定位治疗和对照，以防止结果的误读，并提高研究结果对人类的可转化性。
