Graduate Program in Dentistry, Cruzeiro do Sul University. São Paulo-SP, Brazil.
Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
Braz Dent J. 2024 May 10;35:e245616. doi: 10.1590/0103-6440202405616. eCollection 2024.
The aim of this in vitro study was to evaluate the potential of different fluoridated varnishes to inhibit the progression of incipient caries lesions after cariogenic challenge. Seventy-five enamel specimens of bovine teeth were prepared and selected based on the initial surface microhardness (SMH). The specimens were first subjected to artificial demineralization (in buffer solution) after which SMH was re-analyzed (SM1). They were then randomly assigned to five experimental groups: 1- CONTROL (pH cycling), 2 - MI VAR (MI Varnish with RECALDENTTM - CPP-ACP), 3 - PROFL (Profluorid®), 4 - CLIN (ClinproTM White Varnish with TCP), and 5 - DUR (Duraphat®) (n=15). The varnishes were applied in a thin layer and the specimens were then subjected to pH cycling for eight days. The SMH and cross-sectional microhardness (CSMH) were then analyzed (SM2). The fluoride and calcium ion concentrations in the solution were analyzed by the indirect method and atomic absorption spectrophotometry, respectively. Data were statistically analyzed by Student's t-test, ANOVA/Tukey-Kramer, or Kruskall-Wallis/Dunn tests for individual comparisons (p˂0.05). All varnishes led to significantly higher surface and subsurface remineralization compared with the control group but did not differ from each other. The varnishes with the highest fluoride release were: PROFL and CLIN, followed by MI VAR and DUR. The varnishes with significantly higher release of calcium were: DUR, CLIN, and PROFL. In conclusion, all commercial fluoridated varnishes tested have good potential to inhibit the progression of demineralization, regardless of the ion release mechanisms.
本体外研究旨在评估不同含氟涂料在致龋挑战后抑制初期龋损进展的潜力。将 75 个牛牙釉质标本根据初始表面显微硬度（SMH）进行制备和选择。这些标本首先在缓冲液中进行人工脱矿化（在缓冲液中），然后重新分析 SMH（SM1）。然后将它们随机分配到五个实验组：1- CONTROL（pH 循环）、2 - MI VAR（含 RECALDENTTM-CPP-ACP 的 MI 涂料）、3 - PROFL（Profluorid®）、4 - CLIN（含 TCP 的 ClinproTM 白色涂料）和 5 - DUR（Duraphat®）（n=15）。将涂料涂成薄层，然后将标本进行 pH 循环 8 天。然后分析 SMH 和横截面显微硬度（CSMH）（SM2）。通过间接法和原子吸收分光光度法分别分析溶液中的氟离子和钙离子浓度。通过学生 t 检验、方差分析/Tukey-Kramer 或 Kruskal-Wallis/Dunn 检验进行个体比较（p<0.05）对数据进行统计学分析。与对照组相比，所有涂料均导致表面和次表面再矿化显著增加，但彼此之间无差异。氟释放量最高的涂料是：PROFL 和 CLIN，其次是 MI VAR 和 DUR。钙释放量显著较高的涂料是：DUR、CLIN 和 PROFL。总之，无论离子释放机制如何，所有测试的商业含氟涂料都具有很好的抑制脱矿化进展的潜力。
