Roos E, La Rivière G, Collard J G, Stukart M J, De Baetselier P
Cancer Res. 1985 Dec;45(12 Pt 1):6238-43.
BW5147 lymphoma cells, which are noninvasive and nonmetastatic, were fused with normal T-lymphocytes. The invasiveness of the generated T-cell hybridomas was tested in hepatocyte cultures, and their metastatic potential was tested by tail vein injection. A total of 29 hybridomas generated from alloantigen-activated T-cells were all found to be invasive. One of these cell lines rapidly lost invasiveness in culture. Most hybridomas generated from nonstimulated spleen T-cells were also invasive, but 5 of 27 were not. Six invasive and four noninvasive hybridomas were injected into the tail vein of syngeneic mice. All invasive cell lines caused extensive and widespread tumor growth, particularly in the liver, which was usually severalfold enlarged; the spleen; kidneys; and ovaries. In contrast the noninvasive hybrids, which were tumorigenic upon s.c. injection, did not form any metastases. We conclude that properties derived from normal T-cells, when introduced into noninvasive T-lymphoma cells, cause them to become invasive as well as metastatic. Furthermore for this tumor cell type invasiveness as measured in hepatocyte cultures appears to be closely associated with the ability to colonize organs from the bloodstream.
将非侵袭性和非转移性的BW5147淋巴瘤细胞与正常T淋巴细胞进行融合。在肝细胞培养物中测试所产生的T细胞杂交瘤的侵袭性，并通过尾静脉注射测试其转移潜能。总共29个由同种异体抗原激活的T细胞产生的杂交瘤均被发现具有侵袭性。其中一个细胞系在培养过程中迅速丧失侵袭性。大多数由未受刺激的脾脏T细胞产生的杂交瘤也具有侵袭性，但27个中有5个不具有侵袭性。将6个侵袭性和4个非侵袭性杂交瘤注射到同基因小鼠的尾静脉中。所有侵袭性细胞系均导致广泛的肿瘤生长，尤其是在肝脏，肝脏通常会增大几倍；还有脾脏、肾脏和卵巢。相比之下，非侵袭性杂交瘤在皮下注射时具有致瘤性，但未形成任何转移灶。我们得出结论，当将源自正常T细胞的特性引入非侵袭性T淋巴瘤细胞时，会使它们变得具有侵袭性和转移性。此外，对于这种肿瘤细胞类型，在肝细胞培养物中测得的侵袭性似乎与从血液中定植器官的能力密切相关。
