De Baetselier P, Gorelik E, Eshhar Z, Ron Y, Katzav S, Feldman M, Segal S
J Natl Cancer Inst. 1981 Nov;67(5):1079-87.
The P3-NSI/1-Ag4-1 (NSI) plasmacytoma, when transplanted sc in syngeneic BALB/c mice, grows locally without forming spontaneous metastases. We tested whether somatic hybridization of the NSI cells with spleen B-lymphocytes would render them metastatic in (C57BL/6 X BALB/c) F1 mice. We found that the hybridomas thus produced generated spontaneous metastases with distinct organ specificities. Some hybridomas produced metastases in both liver and spleen, whereas others produced metastases in only the liver. Cells derived from spleen-and liver-seeking hybridomas, when transplanted sc, produced tumors that metastasized to both the liver and spleen. Tumor cells derived from spleen metastasis produced, on transplantation, a tumor that generated spleen metastasis of a larger mass than did tumors derived from liver metastases. Cells derived from liver-seeking hybridomas metastasized to only the liver. Similar patterns of organ specificity were observed after iv injection of the hybridoma cells. The spleen seemed to play determining role in controlling the production of liver metastases by hybridomas that produced both liver and spleen metastases. Such hybridomas did not produce liver metastases when injected into splenectomized recipients. Hybridomas that were only liver-seeking did produce metastases in splenectomized recipients.
P3-NSI/1-Ag4-1（NSI）浆细胞瘤在同基因BALB/c小鼠体内皮下移植时，会在局部生长，不会形成自发转移灶。我们测试了NSI细胞与脾脏B淋巴细胞的体细胞杂交是否会使其在（C57BL/6×BALB/c）F1小鼠中发生转移。我们发现，由此产生的杂交瘤会产生具有明显器官特异性的自发转移灶。一些杂交瘤在肝脏和脾脏中都产生转移灶，而另一些只在肝脏中产生转移灶。源自趋向脾脏和肝脏的杂交瘤的细胞，皮下移植后会产生转移至肝脏和脾脏的肿瘤。源自脾脏转移灶的肿瘤细胞移植后产生的肿瘤，其脾脏转移灶的体积比源自肝脏转移灶的肿瘤产生的转移灶更大。源自趋向肝脏的杂交瘤的细胞只转移至肝脏。静脉注射杂交瘤细胞后也观察到了类似的器官特异性模式。脾脏似乎在控制同时产生肝脏和脾脏转移灶的杂交瘤的肝转移灶形成方面起决定性作用。将此类杂交瘤注射到脾切除的受体中时，不会产生肝转移灶。只趋向肝脏的杂交瘤在脾切除的受体中确实会产生转移灶。
