Drouet X, Charbord P, Neel H, Baillou C, Parmentier C, Najman A, Duhamel G
Exp Hematol. 1985 Dec;13(11):1133-7.
Human granulocyte colony formation has been observed in serum-free methylcellulose cultures with Iscove medium, delipidated bovine serum albumin, iron-saturated transferrin, alpha-thioglycerol, oleylpalmitoyl lecithin, cholesterol, and linoleic acid using serum-free human placental-conditioned medium (SF-HPCM) as the source of colony stimulating factor (CSF). Dose-response curves for SF-HPCM indicated a lower sensitivity to colony-stimulating activity in serum-free cultures than in serum-dependent cultures. Gel filtration of SF-HPCM revealed that CSF fractions with molecular weights in the range of 30 kD are inefficient in serum-free cultures, while fractions with molecular weights in the range of 40 kD stimulate granulocyte colony formation in both types of cultures. These results demonstrate that serum constituents modulate the effects of one of the stimulating factors for granulocyte colony formation, and that serum-free culture conditions are essential for establishing the growth factor requirements of the granulocyte lineage.
在含有伊斯科夫培养基、脱脂牛血清白蛋白、铁饱和转铁蛋白、α-硫甘油、油酰棕榈酰卵磷脂、胆固醇和亚油酸的无血清甲基纤维素培养物中，使用无血清人胎盘条件培养基（SF-HPCM）作为集落刺激因子（CSF）的来源，观察到了人粒细胞集落形成。SF-HPCM的剂量反应曲线表明，无血清培养物对集落刺激活性的敏感性低于血清依赖性培养物。对SF-HPCM进行凝胶过滤显示，分子量在30 kD范围内的CSF组分在无血清培养物中效率低下，而分子量在40 kD范围内的组分在两种类型的培养物中均能刺激粒细胞集落形成。这些结果表明，血清成分调节粒细胞集落形成刺激因子之一的作用，并且无血清培养条件对于确定粒细胞系的生长因子需求至关重要。
