Ge Feng, Ding Wei, Han Cong, Zhang Limin, Liu Qirui, Zhao Jian, Luo Zhaofeng, Jia Chao, Qu Pengpeng, Zhang Liyun
Clinical School of Obstetrics and Gynecology Center, Tianjin Medical University, Tianjin 301700, China.
Department of Gynecological Oncology, Tianjin Central Hospital of Gynecology and Obstetrics, Tianjin 300100, China.
ACS Sens. 2024 Jun 28;9(6):2897-2906. doi: 10.1021/acssensors.3c02776. Epub 2024 May 22.
Ovarian cancer (OC) has the highest mortality rate among malignant tumors, primarily because it is difficult to diagnose early. Exosomes, a type of extracellular vesicle rich in parental information, have garnered significant attention in the field of cancer diagnosis and treatment. They play an important regulatory role in the occurrence, development, and metastasis of OC. Consequently, exosomes have emerged as noninvasive biomarkers for early cancer detection. Therefore, identifying cancer-derived exosomes may offer a novel biomarker for the early detection of OC. In this study, we developed a metal-organic frameworks assembled "double hook"-type aptamer electrochemical sensor, which enables accurate early diagnosis of OC. Under optimal experimental conditions, electrochemical impedance spectroscopy technology demonstrated a good linear relationship within the concentration range of 31-3.1 × 10 particles per microliter, with a detection limit as low as 12 particles per microliter. The universal exosome detection platform is constructed, and this platform can not only differentiate between high-grade serous ovarian cancer (HGSOC) patients and healthy individuals but also distinguish between HGSOC patients and nonhigh-grade serous OC (non-HGSOC). Consequently, it provides a novel strategy for the early diagnosis of OC and holds great significance in clinical differential diagnosis.
卵巢癌（OC）在恶性肿瘤中死亡率最高，主要原因是其难以早期诊断。外泌体是一种富含亲本信息的细胞外囊泡，在癌症诊断和治疗领域备受关注。它们在OC的发生、发展和转移中发挥着重要的调节作用。因此，外泌体已成为癌症早期检测的非侵入性生物标志物。因此，识别癌症来源的外泌体可能为OC的早期检测提供一种新型生物标志物。在本研究中，我们开发了一种金属有机框架组装的“双钩”型适配体电化学传感器，可实现OC的准确早期诊断。在最佳实验条件下，电化学阻抗谱技术在每微升31-3.1×10个颗粒的浓度范围内呈现良好的线性关系，检测限低至每微升12个颗粒。构建了通用的外泌体检测平台，该平台不仅可以区分高级别浆液性卵巢癌（HGSOC）患者和健康个体，还可以区分HGSOC患者和非高级别浆液性OC（非HGSOC）。因此，它为OC的早期诊断提供了一种新策略，在临床鉴别诊断中具有重要意义。
