Key Laboratory of Hunan Province for Efficient Power System and Intelligent Manufacturing, College of Mechanical and Energy Engineering, Shaoyang University, Shaoyang 422000, China.
Key Laboratory of Hunan Province for Efficient Power System and Intelligent Manufacturing, College of Mechanical and Energy Engineering, Shaoyang University, Shaoyang 422000, China.
Int J Biol Macromol. 2024 Jun;271(Pt 2):132530. doi: 10.1016/j.ijbiomac.2024.132530. Epub 2024 May 21.
Synergistically improving the mechanical and degradable properties of polylactic acid (PLA) scaffolds and endowing them with bioactivity are urgent problems to be solved in deepening their application in tissue engineering. In this work, tetracalcium phosphate (TTCP) and porous iron (pFe) were compounded by stirring and vacuum negative pressure, and then they were blended with polylactic acid and a porous scaffold (named TTCP@pFe/PLA) was prepared by selective laser sintering. On the one hand, molten polylactic acid penetrates the pores of porous iron to form an interlocking network, thereby achieving mechanical strengthening. On the other hand, the alkaline environment generated by the dissolution of tetracalcium phosphate can effectively catalyze the hydrolysis of polylactic acid to accelerate the degradation. Meanwhile, the dissolution of tetracalcium phosphate forms a local calcium-rich microenvironment, which rapidly induces apatite formation, that is, confers bioactivity on scaffolds. As a result, the TTCP@pFe/PLA scaffold exhibited a notable enhancement in mechanical strength, being 2.2 times stronger compared to the polylactic acid scaffold. More importantly, MC3T3E1 cells exhibit good adhesion, stretching, and proliferation on the composite scaffold, demonstrating good cytocompatibility. All these good properties of the TTCP@pFe/PLA scaffold indicate that it has potential applications as a novel alternative in bone tissue regeneration.
协同改善聚乳酸（PLA）支架的机械和可降解性能，并赋予其生物活性，是深化其在组织工程中应用所急需解决的问题。在这项工作中，通过搅拌和真空负压将磷酸四钙（TTCP）和多孔铁（pFe）复合，然后与聚乳酸混合，通过选择性激光烧结制备多孔支架（命名为 TTCP@pFe/PLA）。一方面，熔融聚乳酸渗透到多孔铁的孔隙中形成联锁网络，从而实现机械增强。另一方面，磷酸四钙的溶解产生的碱性环境可以有效地催化聚乳酸的水解，从而加速降解。同时，磷酸四钙的溶解形成局部富钙微环境，迅速诱导磷灰石形成，即赋予支架生物活性。结果，TTCP@pFe/PLA 支架的机械强度显著增强，比聚乳酸支架强 2.2 倍。更重要的是，MC3T3E1 细胞在复合支架上表现出良好的黏附、拉伸和增殖，表现出良好的细胞相容性。TTCP@pFe/PLA 支架的所有这些良好性能表明，它具有作为骨组织再生的新型替代物的潜力。
