Laboratory of Biomaterials and Oral Pathology, School of Dentistry, Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
Laboratory of Biomaterials and Oral Pathology, School of Dentistry, Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy; Endodontic Clinical Section, School of Dentistry, Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
Mater Sci Eng C Mater Biol Appl. 2018 Jan 1;82:163-181. doi: 10.1016/j.msec.2017.08.040. Epub 2017 Aug 12.
Polylactic acid (PLA), dicalcium phosphate dihydrate (DCPD) and/or hydraulic calcium silicate (CaSi) have been used to prepare highly-porous scaffolds by thermally induced phase separation technique (TIPS). Three experimental mineral-doped formulations were prepared (PLA-10CaSi, PLA-5CaSi-5DCPD, PLA-10CaSi-10DCPD). Pure PLA scaffolds constituted the control group. Scaffolds were tested for their chemical-physical and biological properties, namely calcium release, alkalinizing activity, surface microchemistry and micromorphology by ESEM, apatite-forming ability by EDX, micro-Raman and IR spectroscopy, thermal properties by differential scanning calorimetry, mechanical properties by quasi-static parallel-plates compression testing, porosity by a standard water-absorption method and direct-contact cytotoxicity. All mineral-doped scaffolds released biologically relevant ions (biointeractive). A B-type carbonated apatite layer (thickness decreasing along the series PLA-10CaSi-10DCPD>PLA-10CaSi>PLA-5CaSi-5DCPD>PLA) was detected on the surface of all the 28d-aged scaffolds. Surface pores of fresh scaffolds ranged from 10 to 20μm in pure PLA to 10-100μm in PLA-10CaSi. An increase in porosity was detected in 28d-aged pure PLA scaffolds (approx. 30% of material loss with decrease of the PLA chain length); differently, in mineral-doped scaffolds, the PLA degradation was balanced by deposition/nucleation of apatite. All scaffolds showed absence of toxicity, in particular PLA-10CaSi-10DCPD. The designed scaffolds are biointeractive (release biologically relevant ions), nucleate apatite, possess high surface and internal open porosity and can be colonized by cells, appearing interesting materials for bone regeneration.
聚乳酸（PLA）、二水合磷酸二氢钙（DCPD）和/或水合硅酸钙（CaSi）已被用于通过热致相分离技术（TIPS）制备高多孔支架。制备了三种实验矿物掺杂配方（PLA-10CaSi、PLA-5CaSi-5DCPD、PLA-10CaSi-10DCPD）。纯 PLA 支架构成对照组。通过 EDX、微拉曼和红外光谱法测试支架的化学物理和生物特性，包括钙释放、碱化活性、表面微观化学和 ESEM 微形貌、磷灰石形成能力、热性能通过差示扫描量热法、通过准静态平行板压缩测试的机械性能、通过标准吸水率法的孔隙率和直接接触细胞毒性。所有矿物掺杂的支架都释放出具有生物相关性的离子（生物相互作用）。在所有 28d 龄期的支架表面都检测到 B 型碳酸磷灰石层（厚度沿 PLA-10CaSi-10DCPD>PLA-10CaSi>PLA-5CaSi-5DCPD>PLA 系列逐渐减少）。新鲜支架表面的孔隙率在纯 PLA 中为 10-20μm，在 PLA-10CaSi 中为 10-100μm。在纯 PLA 支架中检测到 28d 龄期的孔隙率增加（约 30%的材料损失，PLA 链长缩短）；不同的是，在矿物掺杂的支架中，磷灰石的沉积/成核平衡了 PLA 的降解。所有支架均无毒性，特别是 PLA-10CaSi-10DCPD。设计的支架具有生物相互作用（释放具有生物相关性的离子）、磷灰石成核、具有高的表面和内部开放孔隙率、可以被细胞定植，是具有前景的骨再生材料。
