Bos J L, Osinga K A, Van der Horst G, Borst P
Nucleic Acids Res. 1979 Jul 25;6(10):3255-66. doi: 10.1093/nar/6.10.3255.
We have determined the nucleotide sequence of a segment of Saccharomyces cerevisiae mtDNA that contains the structural genes for a cysteine-tRNA and a histidine-tRNA. The genes are approximately 85 bp apart, they do not contain intervening sequences or sequences coding for the 3'-CCA terminus and they are surrounded by nearly pure AT segments. The tRNAs deduced are very AT-rich, 74 and 75 nucleotides long, respectively, and contain one or more unusual features not found in tRNAs from other sources.
我们已经确定了一段酿酒酵母线粒体DNA(mtDNA)的核苷酸序列,该序列包含一个半胱氨酸转运RNA(tRNA)和一个组氨酸转运RNA的结构基因。这两个基因相距约85碱基对(bp),它们不包含间隔序列或编码3'-CCA末端的序列,并且被几乎纯的AT片段所包围。推导得出的tRNA富含AT,分别有74和75个核苷酸长,并且含有一个或多个在其他来源的tRNA中未发现的异常特征。