Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, 1838 Guangzhou North Road, Guangzhou, 510515, Guangdong, People's Republic of China.
Aesthetic Plast Surg. 2024 Oct;48(19):4014-4023. doi: 10.1007/s00266-024-04062-6. Epub 2024 May 22.
In plastic surgery tissue transplantation, tissue ischemia limits transplanted tissue survival. Adipose-derived stem cells (ASCs) and stromal vascular fraction (SVF) show potential for promoting angiogenesis and rescuing ischemic conditions. However, when SVF and ASC suspensions are utilized without the protection of extracellular matrix, the retention rate of transplanted cells tends to be diminished, leading to an unsatisfactory therapeutic outcome. To overcome this, adipose tissue-derived microvascular fragments (ad-MVFs) have emerged as a promising solution.
We conducted enzymatic digestion on human adipose tissue to generate ad-MVFs. These fragments underwent a thorough characterization process, utilizing electron microscopy to assess their structural attributes and enabling a detailed analysis of their intricate morphology. Furthermore, our team investigated the cellular composition of these microvascular fragments, subsequently confirming their ability to enhance the viability of ischemic skin flaps.
The resulting product primarily comprised fragments with sizes ranging from 20 to 50 µm, and some exhibited a sophisticated network-like structure. Electron microscopy examination revealed the presence of collagen components in the product. Additionally, flow cytometry analysis indicated a substantial abundance of adipose-derived stem cells and endothelial cells within these microvascular fragments. Significantly, when tested in treating an ischemic skin flap in a nude mouse model, the product exhibited superior therapeutic efficacy compared to SVF cell suspension.
We have successfully generated human ad-MVFs and established standardized procedures. Compared with SVF, Ad-MVFs have a better effect in the treatment of ischemic diseases.
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在整形外科学的组织移植中，组织缺血会限制移植组织的存活。脂肪来源的干细胞（ASCs）和基质血管部分（SVF）显示出促进血管生成和拯救缺血状态的潜力。然而，当 SVF 和 ASC 悬浮液在没有细胞外基质保护的情况下使用时，移植细胞的保留率往往会降低，导致治疗效果不理想。为了解决这个问题，脂肪组织衍生的微血管片段（ad-MVFs）应运而生。
我们对人脂肪组织进行酶消化以产生 ad-MVFs。这些片段经过彻底的特征描述过程，利用电子显微镜评估它们的结构属性，并对其复杂形态进行详细分析。此外，我们的团队研究了这些微血管片段的细胞组成，随后证实了它们能够增强缺血性皮瓣的活力。
所得产物主要由大小在 20 至 50μm 之间的片段组成，有些片段呈现出复杂的网状结构。电子显微镜检查显示产物中存在胶原成分。此外，流式细胞术分析表明，这些微血管片段中存在大量的脂肪来源干细胞和内皮细胞。重要的是，在裸鼠模型中治疗缺血性皮瓣的实验中，与 SVF 细胞悬浮液相比，该产物表现出更好的治疗效果。
我们已经成功地生成了人 ad-MVFs，并建立了标准化程序。与 SVF 相比，Ad-MVFs 在治疗缺血性疾病方面具有更好的效果。
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