Wu Zengxiang, Yang Yi, Li Tingyao, Shen Zhuo, Zhou Xuan, Zhang Yan
Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640 Guangdong China.
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510640 Guangdong China.
3 Biotech. 2024 Jun;14(6):160. doi: 10.1007/s13205-024-04005-7. Epub 2024 May 20.
vegetables exhibit pronounced heterosis; nevertheless, investigations on fertility-related genes are scarce. The present study scrutinized a recessive genic male-sterile line, 7-3A, capable of generating a completely sterile population, holding significant promise for flowering Chinese cabbage breeding. By whole-genome resequencing of sterile and fertile plants, the male-sterile gene was confined to approximately 185 kb on chromosome A07, situated between markers C719 and NP10 in var. Chiifu-401. Notably, substantial structural variation was identified within this region across diverse reference genomes. Despite discernible expression level disparities of a homologous gene, , between male sterile and fertile plants, no sequence divergence was detected. Further elucidation is required to pinpoint a novel sterile gene within the candidate interval. This investigation contributes to the advancement of both the molecular-assisted breeding scheme for flowering Chinese cabbage and the comprehension of male sterility mechanisms.
The online version contains supplementary material available at 10.1007/s13205-024-04005-7.
蔬菜表现出明显的杂种优势；然而，关于育性相关基因的研究却很少。本研究对一个隐性核雄性不育系7-3A进行了详细研究，该不育系能够产生完全不育的群体，对大白菜育种具有重要意义。通过对不育和可育植株进行全基因组重测序，将雄性不育基因定位在A07染色体上大约185 kb的区域内，该区域位于品种Chiifu-401中标记C719和NP10之间。值得注意的是，在不同参考基因组的该区域内发现了大量结构变异。尽管雄性不育和可育植株之间同源基因的表达水平存在明显差异，但未检测到序列差异。需要进一步阐明以在候选区间内确定一个新的不育基因。本研究有助于推进大白菜分子辅助育种计划以及对雄性不育机制的理解。
在线版本包含可在10.1007/s13205-024-04005-7获取的补充材料。
