• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

高色素密集细胞群中的有丝分裂象能否作为高级别鳞状上皮内病变的细胞诊断标准？

Can Mitotic Figures in Hyperchromatic Crowded Groups be Cytodiagnostic Criteria for High-Grade Squamous Intra-epithelial Lesions?

作者信息

Suzuki Hisae, Kondo Yumeno, Oda Chihiro, Nishikawa Takeshi, Takeuchi Mao, Tatsumi Shigenobu, Hosokawa Sho, Irino Satoshi, Uchiyama Tomoko, Fujii Tomomi, Norimatsu Yoshiaki

机构信息

Department of Pathology, Nara Medical University Hospital, Nara, Japan.

Department of Medical Technology, The Graduate School of Ehime Prefectural University of Health Sciences, Ehime, Japan.

出版信息

J Cytol. 2024 Apr-Jun;41(2):116-122. doi: 10.4103/joc.joc_156_23. Epub 2024 Apr 24.

DOI:10.4103/joc.joc_156_23
PMID:38779606
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11108039/
Abstract

AIMS

The present study aimed to investigate whether the presence of mitoses in hyperchromatic crowded groups (HCGs) in cervical cytological specimens can serve as cytological criteria for high-grade squamous intra-epithelial lesions (HSILs).

METHODS AND MATERIAL

Various parameters were examined, including the frequency of mitotic figures per high power field (HPF) in Pap, hematoxylin eosin (HE) samples, and PHH3 immunocytochemical (ICC) and immunohistochemical (IHC) analyses.

RESULTS

In the Pap and PHH3-ICC samples, the number of mitotic figures observed in HCGs was significantly higher in HSIL ( < 0.001) compared to other groups. Furthermore, the frequency of observing two or more mitoses was significantly higher in HSIL (Pap: = 0.002, PHH3-ICC: < 0.001) than in low-grade squamous intra-epithelial lesions (LSILs). Moreover, a comparison between Pap samples and PHH3-ICC showed that the frequency of two or more mitoses was significantly higher in the PHH3-ICC analysis of HSIL ( = 0.042). Regarding HE and PHH3-IHC samples, counting the number of mitoses in the lower and middle/upper layers of the squamous epithelial layer revealed that HSIL had a significantly higher value (HE: = 0.0089, PHH3-IHC: = 0.0002) than LSIL in the middle/upper layers.

CONCLUSIONS

Hence, the presence of two or more mitotic figures in HCGs per HPF in cervical cytology indicates a suspicion of HSIL. The detection of mitoses in PHH3-ICC samples is more sensitive and easier to observe than in Pap samples, making it a valuable mitotic marker.

摘要

目的

本研究旨在探讨宫颈细胞学标本中深染拥挤细胞群（HCGs）内有丝分裂的存在是否可作为高级别鳞状上皮内病变（HSILs）的细胞学标准。

方法和材料

检查了各种参数，包括巴氏涂片、苏木精伊红（HE）样本中每高倍视野（HPF）有丝分裂象的频率，以及磷酸化组蛋白H3（PHH3）免疫细胞化学（ICC）和免疫组织化学（IHC）分析。

结果

在巴氏涂片和PHH3-ICC样本中，与其他组相比，HSIL中在HCGs中观察到的有丝分裂象数量显著更高（<0.001）。此外，HSIL中观察到两个或更多有丝分裂的频率显著高于低级别鳞状上皮内病变（LSILs）（巴氏涂片：=0.002，PHH3-ICC：<0.001）。而且，巴氏涂片样本与PHH3-ICC之间的比较表明，在HSIL的PHH3-ICC分析中两个或更多有丝分裂的频率显著更高（=0.042）。关于HE和PHH3-IHC样本，对鳞状上皮层下层和中层/上层的有丝分裂数量进行计数发现，HSIL在中层/上层的值显著高于LSIL（HE：=0.0089，PHH3-IHC：=0.0002）。

结论

因此，宫颈细胞学中每个HPF的HCGs内存在两个或更多有丝分裂象提示怀疑HSIL。与巴氏涂片样本相比，在PHH3-ICC样本中检测有丝分裂更敏感且更易于观察，使其成为一种有价值的有丝分裂标记物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e35/11108039/05c7d0e1279c/JCytol-41-116-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e35/11108039/59e87c8fe539/JCytol-41-116-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e35/11108039/bb0210fb628b/JCytol-41-116-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e35/11108039/7f3481661f8f/JCytol-41-116-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e35/11108039/31f7e074c89d/JCytol-41-116-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e35/11108039/4eb6c1a1ba5c/JCytol-41-116-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e35/11108039/05c7d0e1279c/JCytol-41-116-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e35/11108039/59e87c8fe539/JCytol-41-116-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e35/11108039/bb0210fb628b/JCytol-41-116-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e35/11108039/7f3481661f8f/JCytol-41-116-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e35/11108039/31f7e074c89d/JCytol-41-116-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e35/11108039/4eb6c1a1ba5c/JCytol-41-116-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e35/11108039/05c7d0e1279c/JCytol-41-116-g006.jpg

相似文献

1
Can Mitotic Figures in Hyperchromatic Crowded Groups be Cytodiagnostic Criteria for High-Grade Squamous Intra-epithelial Lesions?高色素密集细胞群中的有丝分裂象能否作为高级别鳞状上皮内病变的细胞诊断标准？
J Cytol. 2024 Apr-Jun;41(2):116-122. doi: 10.4103/joc.joc_156_23. Epub 2024 Apr 24.
2
The association of atypical squamous cells, cannot exclude a high grade squamous intraepithelial lesion, hyperchromatic crowded groups and high grade squamous intraepithelial lesions involving endocervical glands.非典型鳞状细胞不能排除高级别鳞状上皮内病变，伴有深染拥挤的细胞团和累及宫颈腺体的高级别鳞状上皮内病变。
Diagn Cytopathol. 2021 Sep;49(9):1008-1011. doi: 10.1002/dc.24805. Epub 2021 May 21.
3
Improving mitotic cell counting accuracy and efficiency using phosphohistone-H3 (PHH3) antibody counterstained with haematoxylin and eosin as part of breast cancer grading.利用磷酸化组蛋白 H3（PHH3）抗体与苏木精和伊红复染，提高有丝分裂细胞计数的准确性和效率，作为乳腺癌分级的一部分。
Histopathology. 2023 Feb;82(3):393-406. doi: 10.1111/his.14837. Epub 2022 Nov 18.
4
Mitotic count by phosphohistone H3 immunohistochemical staining predicts survival and improves interobserver reproducibility in well-differentiated neuroendocrine tumors of the pancreas.磷酸组蛋白 H3 免疫组化染色的有丝分裂计数可预测生存并提高胰腺高分化神经内分泌肿瘤的观察者间可重复性。
Am J Surg Pathol. 2015 Jan;39(1):13-24. doi: 10.1097/PAS.0000000000000341.
5
Anti-Phosphohistone H3-Positive Mitoses Are Linked to Pathological Response in Neoadjuvantly Treated Breast Cancer.抗磷酸化组蛋白H3阳性有丝分裂与新辅助治疗的乳腺癌的病理反应相关。
Breast Care (Basel). 2017 Sep;12(4):244-250. doi: 10.1159/000463377. Epub 2017 Aug 2.
6
Cytomorphological Features of Hyperchromatic Crowded Groups in Liquid-Based Cervicovaginal Cytology: A Single Institutional Experience.液基宫颈阴道细胞学中深染拥挤细胞群的细胞形态学特征：单机构经验
J Pathol Transl Med. 2019 Nov;53(6):393-398. doi: 10.4132/jptm.2019.08.14. Epub 2019 Sep 16.
7
Value of counting positive PHH3 cells in the diagnosis of uterine smooth muscle tumors.计数阳性PHH3细胞在子宫平滑肌肿瘤诊断中的价值。
Int J Clin Exp Pathol. 2015 May 1;8(5):4418-26. eCollection 2015.
8
Assessment and prognostic significance of mitotic index using the mitosis marker phospho-histone H3 in low and intermediate-grade infiltrating astrocytomas.使用有丝分裂标记物磷酸化组蛋白H3评估低级别和中级浸润性星形细胞瘤的有丝分裂指数及其预后意义。
Am J Surg Pathol. 2006 May;30(5):657-64. doi: 10.1097/01.pas.0000202048.28203.25.
9
Sensitivity and usefulness of anti-phosphohistone-H3 antibody immunostaining for counting mitotic figures in meningioma cases.抗磷酸化组蛋白H3抗体免疫染色在脑膜瘤病例有丝分裂计数中的敏感性和实用性。
Brain Tumor Pathol. 2009;26(2):51-7. doi: 10.1007/s10014-009-0249-9. Epub 2009 Oct 27.
10
Prognostic importance of mitosis quantification and PHH3 expression in oral epithelial dysplasia.在口腔上皮异型增生中，有丝分裂计数和 PHH3 表达的预后意义。
Virchows Arch. 2024 Jan;484(1):47-59. doi: 10.1007/s00428-023-03668-6. Epub 2023 Oct 26.

引用本文的文献

1
Quantitative Structural Analysis of Hyperchromatic Crowded Cell Groups in Cervical Cytology: Overcoming Diagnostic Pitfalls.宫颈细胞学中嗜碱性浓集细胞群的定量结构分析：克服诊断陷阱
Cancers (Basel). 2024 Dec 21;16(24):4258. doi: 10.3390/cancers16244258.

本文引用的文献

1
A Practical Approach to Squamous Abnormalities on Cervical Cytology: Overview of Interpretive Criteria and Guidance for Altering Thresholds in Response to Quality Assurance Findings.宫颈细胞学鳞状上皮异常的实用处理方法：解释标准概述及根据质量保证结果调整阈值的指南
Acta Cytol. 2023;67(2):129-142. doi: 10.1159/000528531. Epub 2023 Jan 26.
2
Cervical Pre-cancers: Biopsy and Immunohistochemistry.宫颈上皮内瘤变：活检与免疫组织化学
Cytojournal. 2022 Jun 14;19:38. doi: 10.25259/CMAS_03_13_2021. eCollection 2022.
3
Squamous intraepithelial lesions (SIL: LSIL, HSIL, ASCUS, ASC-H, LSIL-H) of Uterine Cervix and Bethesda System.
子宫颈鳞状上皮内病变（SIL：低度鳞状上皮内病变、高度鳞状上皮内病变、非典型鳞状细胞意义不明确、非典型鳞状细胞不排除高度病变、低度鳞状上皮内病变伴高危型HPV感染）与贝塞斯达系统
Cytojournal. 2021 Jul 17;18:16. doi: 10.25259/Cytojournal_24_2021. eCollection 2021.
4
Cytomorphological Features of Hyperchromatic Crowded Groups in Liquid-Based Cervicovaginal Cytology: A Single Institutional Experience.液基宫颈阴道细胞学中深染拥挤细胞群的细胞形态学特征：单机构经验
J Pathol Transl Med. 2019 Nov;53(6):393-398. doi: 10.4132/jptm.2019.08.14. Epub 2019 Sep 16.
5
Anti-Phosphohistone H3-Positive Mitoses Are Linked to Pathological Response in Neoadjuvantly Treated Breast Cancer.抗磷酸化组蛋白H3阳性有丝分裂与新辅助治疗的乳腺癌的病理反应相关。
Breast Care (Basel). 2017 Sep;12(4):244-250. doi: 10.1159/000463377. Epub 2017 Aug 2.
6
The mitosis-specific marker phosphohistone-H3 (PHH3) is an independent prognosticator in uterine smooth muscle tumours: an outcome-based study.有丝分裂特异性标志物磷酸组蛋白-H3（PHH3）是子宫平滑肌肿瘤的独立预后因子：基于结局的研究。
Histopathology. 2017 Apr;70(5):746-755. doi: 10.1111/his.13124. Epub 2017 Jan 24.
7
Better see to better agree: phosphohistone H3 increases interobserver agreement in mitotic count for meningioma grading and imposes new specific thresholds.看得更仔细才能更好地达成一致：磷酸化组蛋白H3提高了脑膜瘤分级中有丝分裂计数的观察者间一致性，并设定了新的特定阈值。
Neuro Oncol. 2015 May;17(5):663-9. doi: 10.1093/neuonc/nov002. Epub 2015 Feb 1.
8
Evaluation of mitotic activity index in breast cancer using whole slide digital images.利用全切片数字图像评估乳腺癌的有丝分裂活性指数。
PLoS One. 2013 Dec 30;8(12):e82576. doi: 10.1371/journal.pone.0082576. eCollection 2013.
9
Cervical cytology with a diagnosis of atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H): a follow-up study with corresponding histology and significance of predicting dysplasia by human papillomavirus (HPV) DNA testing.细胞学诊断为不典型鳞状细胞，不能排除高级别鳞状上皮内病变（ASC-H）：一项随访研究，包括相应的组织病理学检查以及人乳头瘤病毒（HPV）DNA 检测预测不典型增生的意义。
Arch Gynecol Obstet. 2014 Mar;289(3):645-8. doi: 10.1007/s00404-013-3015-5. Epub 2013 Sep 4.
10
The Lower Anogenital Squamous Terminology Standardization project for HPV-associated lesions: background and consensus recommendations from the College of American Pathologists and the American Society for Colposcopy and Cervical Pathology.人乳头瘤病毒相关病变的下生殖道鳞状上皮术语标准化项目：美国病理学家学会和美国阴道镜和宫颈病理学会的背景和共识建议。
Int J Gynecol Pathol. 2013 Jan;32(1):76-115. doi: 10.1097/PGP.0b013e31826916c7.