一种含有亲电汞和发色离去基团的硫醇标记试剂及反应活性探针。
A thiol-labelling reagent and reactivity probe containing electrophilic mercury and a chromophoric leaving group.
作者信息
Baines B S, Brocklehurst K
出版信息
Biochem J. 1979 Jun 1;179(3):701-4. doi: 10.1042/bj1790701.
Abstract
2-(2'-Pyridylmercapto)mercuri-4-nitrophenol was synthesized and evaluated as a thiol-labelling reagent containing a chromophoric leaving group and as a reactivity probe by studies on its reactions with 2-mercaptoethanol and with papain (EC 3.4.22.2).
摘要
合成了2-(2'-吡啶基巯基)汞-4-硝基苯酚，并通过研究其与2-巯基乙醇和木瓜蛋白酶（EC 3.4.22.2）的反应，将其评估为一种含有发色离去基团的硫醇标记试剂和一种反应活性探针。
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A thiol-labelling reagent and reactivity probe containing electrophilic mercury and a chromophoric leaving group.一种含有亲电汞和发色离去基团的硫醇标记试剂及反应活性探针。
Biochem J. 1979 Jun 1;179(3):701-4. doi: 10.1042/bj1790701.
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Investigation of the catalytic site of actinidin by using benzofuroxan as a reactivity probe with selectivity for the thiolate-imidazolium ion-pair systems of cysteine proteinases. Evidence that the reaction of the ion-pair of actinidin (pKI 3.0, pKII 9.6) is modulated by the state of ionization of a group associated with a molecular pKa of 5.5.以苯并呋咱为反应性探针研究猕猴桃蛋白酶的催化位点，该探针可选择性作用于半胱氨酸蛋白酶的硫醇盐-咪唑鎓离子对体系。有证据表明，猕猴桃蛋白酶（pKI 3.0，pKII 9.6）离子对的反应受一个与分子pKa为5.5的基团的电离状态调节。
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Reactivities of neutral and cationic forms of 2,2'-dipyridyl disulphide towards thiolate anions. Detection of differences between the active centres of actinidin, papain and ficin by a three-protonic-state reactivity probe.2,2'-二吡啶二硫化物的中性和阳离子形式对硫醇盐阴离子的反应活性。通过三质子态反应探针检测猕猴桃蛋白酶、木瓜蛋白酶和无花果蛋白酶活性中心之间的差异。
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The equilibrium assumption is valid for the kinetic treatment of most time-dependent protein-modification reactions.平衡假设对于大多数随时间变化的蛋白质修饰反应的动力学处理是有效的。
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本文引用的文献
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USE OF "REPORTER GROUPS" IN STRUCTURE-FUNCTION STUDIES OF PROTEINS.“报告基团”在蛋白质结构-功能研究中的应用。
Proc Natl Acad Sci U S A. 1964 Oct;52(4):1017-24. doi: 10.1073/pnas.52.4.1017.
2
Stoicheiometric, reactivity and environmental probes for sulphydryl groups in enzymes.
Biochem Soc Symp. 1970;31:39-47.
3
A new class of chromophoric organomercurials and their reactions with D-glyceraldehyde 3-phosphate dehydrogenase.一类新型发色有机汞化合物及其与3-磷酸甘油醛脱氢酶的反应。
Biochem J. 1969 Dec;115(5):913-21. doi: 10.1042/bj1150913.
4
Substrate binding to an active creatine kinase with a thiol-bound mercurinitrophenol chromophoric probe.底物与带有硫醇结合的汞硝基苯酚发色探针的活性肌酸激酶的结合。
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2858-62. doi: 10.1073/pnas.70.10.2858.
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Reactions of L-ergothioneine and some other aminothiones with2,2'-and 4,4'-dipyridyl disulphides and of L-ergothioneine with iodoacetamide. 2-Mercaptoimidazoles, 2- and 4-thiopyridones, thiourea and thioacetamide as highly reactive neutral sulphur nucleophils.L-麦角硫因及其他一些氨基硫酮与2,2'-和4,4'-联吡啶二硫化物的反应以及L-麦角硫因与碘乙酰胺的反应。2-巯基咪唑、2-和4-硫代吡啶酮、硫脲和硫代乙酰胺作为高反应性中性硫亲核试剂。
Biochem J. 1974 Apr;139(1):221-35. doi: 10.1042/bj1390221.
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The reaction of creatine kinase with 2-chloromercuri-4-nitrophenol.肌酸激酶与2-氯汞基-4-硝基苯酚的反应。
J Biol Chem. 1974 Sep 25;249(18):5885-8.
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Spectrochemical and ligand-binding studies of an active mercurinitrophenol-labeled creatine kinase.活性汞硝基苯酚标记的肌酸激酶的光谱化学和配体结合研究。
Biochemistry. 1977 Aug 23;16(17):3838-45. doi: 10.1021/bi00636a019.
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