State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, China.
Hunan Provincial Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha, China.
mSystems. 2024 Jun 18;9(6):e0112423. doi: 10.1128/msystems.01124-23. Epub 2024 May 23.
Plants rely on strigolactones (SLs) to regulate their development and form symbiotic relationships with microbes as part of the adaptive phosphorus (P) efficiency strategies. However, the impact of SLs on root-associated microbial communities in response to P availability remains unknown. Here, root microbiota of SL biosynthesis () and perception () were compared to wild-type Col-0 plants under different P concentrations. Using high-throughput sequencing, the relationship between SLs, P concentrations, and the root-associated microbiota was investigated to reveal the variation in microbial diversity, composition, and interaction. Plant genotypes and P availability played important but different roles in shaping the root-associated microbial community. Importantly, SLs were found to attract in low P conditions, which included an isolated CP-2 () that could promote plant growth in cocultivation experiments. Moreover, SLs could change the topologic structure within co-occurrence networks and increase the number of keystone taxa (e.g., Rhizobiaceae and Acidobacteriaceae) to enhance microbial community stability. This study reveals the key role of SLs in mediating root-associated microbiota interactions.IMPORTANCEStrigolactones (SLs) play a crucial role in plant development and their symbiotic relationships with microbes, particularly in adapting to phosphorus levels. Using high-throughput sequencing, we compared the root microbiota of plants with SL biosynthesis and perception mutants to wild-type plants under different phosphorus concentrations. These results found that SLs can attract beneficial microbes in low phosphorus conditions to enhance plant growth. Additionally, SLs affect microbial network structures, increasing the stability of microbial communities. This study highlights the key role of SLs in shaping root-associated microbial interactions, especially in response to phosphorus availability.
植物依赖 Strigolactones（SLs）来调节其发育并与微生物形成共生关系，这是适应磷（P）效率策略的一部分。然而，SLs 对根相关微生物群落响应 P 可利用性的影响尚不清楚。在这里，与不同 P 浓度下的野生型 Col-0 植物相比，比较了 SL 生物合成（）和感知（）的根微生物群。使用高通量测序，研究了 SLs、P 浓度和根相关微生物群之间的关系，以揭示微生物多样性、组成和相互作用的变化。植物基因型和 P 可利用性在塑造根相关微生物群落方面发挥着重要但不同的作用。重要的是，发现 SLs 在低 P 条件下吸引，其中包括一种可在共培养实验中促进植物生长的分离 CP-2（）。此外，SLs 可以改变共生网络内的拓扑结构，并增加关键分类群（例如 Rhizobiaceae 和 Acidobacteriaceae）的数量，以增强微生物群落的稳定性。这项研究揭示了 SLs 在介导根相关微生物群相互作用中的关键作用。
