Mori Y, Mori T, Ueda S, Yoshida H, Iesato K, Wakashin Y, Wakashin M, Okuda K
Clin Exp Immunol. 1985 Sep;61(3):577-84.
This study was based on our model of experimental autoimmune hepatitis produced by immunizing C57BL/6 (B6) mice with syngeneic liver proteins and Freund's complete adjuvant. Spleen cells obtained from these hepatitis mice were transferred to syngeneic normal recipient mice, and histological changes occurring in the liver and the role of cellular immunity in producing liver damages in recipient mice were investigated. Sensitized spleen cells from these immunized (donor) mice were fractionated by a nylon wool column and injected intravenously into normal B6 mice. Histology of the liver of the recipient mice showed mild infiltration of mononuclear cells around the periportal area 7 days after the transfer of sensitized spleen cells, and changes were most prominent in the mice injected with the fraction of nylon wool column adherent spleen cells. Induction of these liver lesions in the recipient mice was blocked by treatment of sensitized donor spleen cells with anti-Thy 1,2 monoclonal antibody and guinea pig complement before injection. Lymphocyte reactivity to liver-specific lipoprotein (LSP) in recipient mice was studied by a lymphocyte transformation system, and a high immune response was demonstrated with the fraction of nylon wool column non-adherent (T-enriched) spleen cells. These data seem to indicate T-cell interactions between donor and recipient mice in the transfer study using our experimental autoimmune hepatitis model.
本研究基于我们通过用同基因肝蛋白和弗氏完全佐剂免疫C57BL/6（B6）小鼠建立的实验性自身免疫性肝炎模型。从这些肝炎小鼠获得的脾细胞被转移到同基因正常受体小鼠中，研究受体小鼠肝脏中发生的组织学变化以及细胞免疫在产生肝脏损伤中的作用。将这些免疫（供体）小鼠的致敏脾细胞通过尼龙毛柱进行分离，并静脉注射到正常B6小鼠中。在转移致敏脾细胞7天后，受体小鼠肝脏的组织学显示门周区域周围有轻度单核细胞浸润，在注射尼龙毛柱黏附脾细胞组分的小鼠中变化最为明显。在注射前用抗Thy 1,2单克隆抗体和豚鼠补体处理致敏供体脾细胞，可阻断受体小鼠中这些肝脏病变的诱导。通过淋巴细胞转化系统研究受体小鼠对肝特异性脂蛋白（LSP）的淋巴细胞反应性，结果显示尼龙毛柱非黏附（富含T细胞）脾细胞组分具有高免疫反应性。这些数据似乎表明在使用我们的实验性自身免疫性肝炎模型进行的转移研究中，供体和受体小鼠之间存在T细胞相互作用。
