Batista Celso Soares Pereira, Loscos-Giménez Irene, Gámez María, Altaba Raul, de Miniac Daniela, Martí Neus, Bassaganyas Francisca, Juanes Elena, Rivera Alba, Navarro Ferran
Microbiology department, Hospital de la Santa Creu i Sant Pau, C/ Sant Quintí, 89. Planta B-2, Barcelona, 08041, Spain.
Department of Genetics and Microbiology, Autonomous University of Barcelona, Barcelona, Spain.
J Ophthalmic Inflamm Infect. 2024 May 23;14(1):20. doi: 10.1186/s12348-024-00404-2.
Intravitreal injections are a common ophthalmologic procedure. While infections following these injections are rare, they can lead to endophthalmitis, with potentially serious consequences. Various methods have been proposed to prevent endophthalmitis, including the use of antisepsis and antibiotics in patient preparation.
To evaluate the antiseptic efficacy of aqueous chlorhexidine (CHX) and povidone-iodine (PI) when used alone and in combination with lidocaine gel (LG) in vitro.
Two independent experimental trials were conducted. The first trial determined the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of CHX and PI against six bacterial strains. The second trial evaluated the bactericidal efficacy of the antiseptic agents (CHX 0.1% and PI 5%) and their combination with LG against the same bacterial strains.
CHX was more effective than PI in reducing the number of colonies forming units (cfus) of the tested bacteria. The order in which the antiseptic and LG were administered affected their effectiveness, with CHX administered before LG resulting in greater reduction of bacterial growth.
CHX 0.1% is more effective than PI 5% as an antiseptic agent. Application of CHX and PI prior to the use of lidocaine gel results in a more effective reduction of microorganisms.
玻璃体内注射是一种常见的眼科手术。虽然这些注射后感染很少见，但可能导致眼内炎，产生潜在的严重后果。已经提出了各种预防眼内炎的方法，包括在患者准备过程中使用防腐剂和抗生素。
在体外评估单独使用以及与利多卡因凝胶（LG）联合使用时，氯己定水溶液（CHX）和聚维酮碘（PI）的防腐效果。
进行了两项独立的实验性试验。第一项试验确定了CHX和PI对六种细菌菌株的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。第二项试验评估了防腐剂（0.1% CHX和5% PI）及其与LG联合使用对相同细菌菌株的杀菌效果。
在减少受试细菌的菌落形成单位（cfu）数量方面，CHX比PI更有效。防腐剂和LG的给药顺序影响其效果，在LG之前给予CHX可使细菌生长减少得更多。
0.1% CHX作为防腐剂比5% PI更有效。在使用利多卡因凝胶之前应用CHX和PI可更有效地减少微生物。
