Baschong W, Lucocq J M, Roth J
Histochemistry. 1985;83(5):409-11. doi: 10.1007/BF00509201.
Reduction of HAuCl4 by NaSCN or KSCN produces colloidal gold particles of 2.6 nm in diameter and homogeneous in size (coefficient of variation approximately 15%). The AuSCN sol forms protein-gold complexes. The amount of protein required to form an AuSCN-protein complex is best determined in the electron microscope, where serial dilutions of protein with gold sol are inspected for the presence of aggregates. By immuno-electron microscopy SCN-gold complexed to protein A is active and visible as is shown by revealing alpha-amylase in rat pancreatic acinar cells.
用硫氰酸钠（NaSCN）或硫氰酸钾（KSCN）还原氯金酸（HAuCl4）可产生直径为2.6 nm且尺寸均匀的胶体金颗粒（变异系数约为15%）。硫氰酸金溶胶形成蛋白质-金复合物。形成硫氰酸金-蛋白质复合物所需的蛋白量最好在电子显微镜下测定，在电子显微镜中检查蛋白质与金溶胶的系列稀释液中是否存在聚集体。通过免疫电子显微镜观察，与蛋白A复合的硫氰酸金-金复合物具有活性且可见，如在大鼠胰腺腺泡细胞中显示α-淀粉酶的情况所示。
