School of Environmental Science, University of Shiga Prefecture, Hikone, Shiga, Japan.
Faculty of Bioresource Sciences, Akita Prefectural University, Akita, Japan.
Anim Sci J. 2024 Jan-Dec;95(1):e13957. doi: 10.1111/asj.13957.
The purpose of this study was to investigate the time-dependent change in Reishi (Ganoderma lingzhi) triterpenoids in rumen fluid. G. lingzhi fruiting bodies were milled and incubated in a tube with rumen fluid for 0, 4, 8, 12, 24, and 48 h at 39°C. After incubation, all the tubes were freeze-dried and extracted by ethanol. The contents of 18 triterpenoids in the ethanol extract were quantitated by liquid chromatography-mass spectrometry (LC-MS/MS). Based on the results, triterpenoids were categorized into three groups: (1) rapid decrease, indicating reductions of more than 50% within 8 h; (2) mild decrease, with reductions of more than 50% within 48 h; and (3) minimal change, even after 48 h, there was not much change. Ganoderic acid C6, DM, H, K, and TR as well as Ganoderenic acid D were classified in (1); Ganoderic acid LM2 and T-Q as well as Ganoderiol F in (2); and Ganoderic acid A, B, C1, C2, I, and TN; Gnoderenic acid C; and Ganodermanontriol in (3). In addition, a relationship between chemical structure and metabolic speed was observed in some cases. The results of this study revealed that G. lingzhi triterpenoids are digested and metabolized at different speeds in ruminant fluid.
本研究旨在探讨灵芝三萜类化合物在瘤胃液中随时间的变化。将灵芝子实体粉碎后，在试管中与瘤胃液一起于 39°C 孵育 0、4、8、12、24 和 48 小时。孵育后，所有试管均冻干并用乙醇提取。采用液相色谱-质谱联用（LC-MS/MS）定量分析乙醇提取物中 18 种三萜类化合物的含量。结果表明，三萜类化合物可分为三类：（1）快速减少，8 小时内减少 50%以上；（2）温和减少，48 小时内减少 50%以上；（3）变化极小，即使孵育 48 小时后也没有太大变化。灵芝酸 C6、DM、H、K 和 TR 以及 Ganoderic acid D 属于（1）；灵芝酸 LM2 和 T-Q 以及 Ganoderiol F 属于（2）；灵芝酸 A、B、C1、C2、I 和 TN；Ganoderic acid C；以及 Ganodermanontriol 属于（3）。此外，在某些情况下观察到化学结构与代谢速度之间存在关系。本研究结果表明，灵芝三萜类化合物在反刍动物胃液中以不同的速度被消化和代谢。
