Che Xian-Qiang, Li Shao-Ping, Zhao Jing
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China.
Zhongguo Zhong Yao Za Zhi. 2017 May;42(10):1908-1915. doi: 10.19540/j.cnki.cjcmm.20170412.001.
A new triterpenoid and 18 analogues were isolated from the water extract of Ganoderma lucidum by column chromatographic techniques, including silica gel, ODS, Sephadex LH-20, and HPLC. The new compound was elucidated as 2β-acetoxy-3β,25-dihydroxy-7,11,15-trioxo-lanost-8-en-26-oic acid on the basis of analyses of extensive spectroscopic data and its physicochemical properties. Comparison of NMR data with those reported in literature, the known analogues were determined as ganoderic acid H (2), 12β-acetoxy-3β,7β-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid (3), ganoderenic acid D (4),ganoderic acid C1 (5),ganoderic acid G (6),3β,7β-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid (7),ganoderic acid B (8),ganoderic acid C6 (9),3β,15α-dihydroxy-7,11,23-trioxo-lanost-8,16-dien-26-oic acid (10),ganoderic acid A (11),ganolucidic acid A (12),lucidenic acid E2 (13),lucidenic acid N (14),lucidenic acid P (15), lucidenic acid B (16),lucidenic acid A (17),lucidenic acid C (18),and lucidenic acid L (19), respectively. Compound 1 is new compound and compounds 2-19 have been reported from G. lucidum. The present study enriches the knowledge of the chemical constituent of G. lucidum and completes chemical investigation of water decoction that is traditional use of G. lucidum.
采用硅胶柱色谱、ODS柱色谱、葡聚糖凝胶LH - 20柱色谱及高效液相色谱等柱色谱技术，从灵芝水提取物中分离得到1个新的三萜类化合物及18个类似物。通过对大量光谱数据及其理化性质的分析，确定该新化合物为2β - 乙酰氧基 - 3β,25 - 二羟基 - 7,11,15 - 三氧代 - 羊毛甾 - 8 - 烯 - 26 - 酸。通过将核磁共振数据与文献报道的数据进行比较，确定已知类似物分别为灵芝酸H（2）、12β - 乙酰氧基 - 3β,7β - 二羟基 - 11,15,23 - 三氧代 - 羊毛甾 - 8,16 - 二烯 - 26 - 酸（3）、灵芝烯酸D（4）、灵芝酸C1（5）、灵芝酸G（6）、3β,7β - 二羟基 - 11,15,23 - 三氧代 - 羊毛甾 - 8,16 - 二烯 - 26 - 酸（7）、灵芝酸B（8）、灵芝酸C6（9）、3β,15α - 二羟基 - 7,11,23 - 三氧代 - 羊毛甾 - 8,16 - 二烯 - 26 - 酸（10）、灵芝酸A（11）、灵芝酸A（12）、灵芝烯酸E2（13）、灵芝烯酸N（14）、灵芝烯酸P（15）、灵芝烯酸B（16）、灵芝烯酸A（17）、灵芝烯酸C（18）和灵芝烯酸L（19）。化合物1为新化合物，化合物2 - 19已从灵芝中报道过。本研究丰富了灵芝化学成分的知识，并完成了灵芝传统用药水煎液的化学研究。
