College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
College of Agriculture and Biotechnology, Wenzhou Vocational College of Science and Technology (Wenzhou Academy of Agricultural Sciences), Wenzhou, China.
PeerJ. 2024 May 20;12:e17337. doi: 10.7717/peerj.17337. eCollection 2024.
Chinese cabbage ( L. ssp. (L.) Makino) stands as a widely cultivated leafy vegetable in China, with its leaf morphology significantly influencing both quality and yield. Despite its agricultural importance, the precise mechanisms governing leaf wrinkling development remain elusive. This investigation focuses on 'Wutacai', a representative cultivar of the Tacai variety ( L. ssp. var. Tsen et Lee), renowned for its distinct leaf wrinkling characteristics. Within the genome of 'Wutacai', we identified a total of 18 , designated as , revealing their conservation within the genus, and their close homology to in Arabidopsis. Expression profiling unveiled that in Chinese Cabbage exhibited organ-specific and leaf position-dependent variation. Additionally, transcriptome sequencing data from the flat leaf cultivar 'Suzhouqing' and the wrinkled leaf cultivar 'Wutacai' revealed differentially expressed genes (DEGs) related to auxin during the early phases of leaf development, particularly the gene. In summary, this study successfully identified the gene family in 'Wutacai' and elucidated its potential function in leaf wrinkling trait, to provide valuable insights into the prospective molecular mechanisms that regulate leaf wrinkling in Chinese cabbage.
白菜（L. ssp. (L.) Makino）在中国是一种广泛种植的叶菜，其叶片形态对品质和产量有显著影响。尽管具有重要的农业意义，但控制叶片皱缩发育的确切机制仍难以捉摸。本研究以特菜品种（L. ssp. var. Tsen et Lee）的代表品种“乌塌菜”为对象，该品种以其独特的叶片皱缩特征而闻名。在“乌塌菜”的基因组中，我们共鉴定到 18 个 ，命名为 ，表明它们在芸薹属内的保守性，以及与拟南芥中的 具有密切的同源性。表达谱分析表明，芸薹属中的 表现出器官特异性和叶片位置依赖性变化。此外，来自平叶品种“苏州青”和皱叶品种“乌塌菜”的转录组测序数据揭示了在叶片发育早期与生长素相关的差异表达基因（DEGs），特别是 基因。总之，本研究成功鉴定了“乌塌菜”中的 基因家族，并阐明了其在叶片皱缩性状中的潜在功能，为研究调控白菜叶片皱缩的分子机制提供了有价值的线索。
