Department of Horticulture, Shenyang Agricultural University , Shenyang, Liaoning, 110866 , China.
Breed Sci. 2014 Jun;64(2):149-55. doi: 10.1270/jsbbs.64.149. Epub 2014 Jun 1.
To produce hybrid seeds of Wutacai (Brassica campestris L. ssp. chinensis (L.) Makino var. rosularis Tsen et Lee), a "directional transfer program" was designed to breed the multiple-allele male sterile line of Wutacai. A multiple-allele male sterile line of Naibaicai (Brassica campestris L. ssp. chinensis L., S01) was used as the male sterile resource, and an inbred line of Wutacai (WT01) was used as the target line. Recurrent backcrossing was employed to transfer the male sterility and other botanical traits simultaneously, while the genotype was identifiedthrough test crossing. The male sterility was successfully transferred from S01 to WT01. A new male sterile line, GMS-3, with similar botanical traits to WT01, was bred. Four hybrid combinations were generated with GMS-3 as the female parent. One hybrid (C1) that contained the most desirable traits was developed from the new male sterile line.
为了生产乌塌菜（芸薹属芸薹种白菜亚种蕻菜变种）的杂交种子，设计了一个“定向转移程序”来培育乌塌菜的多等位雄性不育系。以内白菜（芸薹属芸薹种白菜亚种，S01）的多等位雄性不育系作为雄性不育资源，以乌塌菜的自交系（WT01）作为目标系。通过回交反复转移雄性不育性和其他植物学特性，同时通过测交鉴定基因型。雄性不育性成功地从 S01 转移到 WT01。培育出了一个具有与 WT01 相似植物学特性的新雄性不育系 GMS-3。用 GMS-3 作为母本生成了四个杂交组合。从新的雄性不育系中培育出了一个具有最理想特性的杂交种（C1）。
