Wu-Chuang Alejandra, Mateos-Hernandez Lourdes, Abuin-Denis Lianet, Maitre Apolline, Avellanet Janet, García Arlem, Fuentes Dasha, Cabezas-Cruz Alejandro
Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, F-94700, France.
Animal Biotechnology Department, Center for Genetic Engineering and Biotechnology, Avenue 31 between 158 and 190, P.O. Box 6162, 10600, Havana, Cuba.
Heliyon. 2024 May 11;10(10):e30914. doi: 10.1016/j.heliyon.2024.e30914. eCollection 2024 May 30.
Breast cancer, a global health concern affecting women, has been linked to alterations in the gut microbiota, impacting various aspects of human health. This study investigates the interplay between breast cancer and the gut microbiome, particularly focusing on colonization resistance-an essential feature of the microbiota's ability to prevent pathogenic overgrowth. Using a mouse model of breast cancer, we employ diversity analysis, co-occurrence network analysis, and robustness tests to elucidate the impact of breast cancer on microbiome dynamics. Our results reveal that breast cancer exposure affects the bacterial community's composition and structure, with temporal dynamics playing a role. Network analysis demonstrates that breast cancer disrupts microbial interactions and decreases network complexity, potentially compromising colonization resistance. Moreover, network robustness analysis shows the susceptibility of the microbiota to node removal, indicating potential vulnerability to pathogenic colonization. Additionally, predicted metabolic profiling of the microbiome highlights the significance of the enzyme EC 6.2.1.2 - Butyrate--CoA ligase, potentially increasing butyrate, and balancing the reduction of colonization resistance. The identification of as a key contributor to this enzyme suggests its role in shaping the microbiota's response to breast cancer. This study uncovers the intricate relationship between breast cancer, the gut microbiome, and colonization resistance, providing insights into potential therapeutic strategies and diagnostic approaches for breast cancer patients.
乳腺癌是一个影响全球女性的健康问题，它与肠道微生物群的改变有关，进而影响人类健康的各个方面。本研究调查了乳腺癌与肠道微生物组之间的相互作用，特别关注定殖抗性——微生物群预防病原体过度生长能力的一个基本特征。利用乳腺癌小鼠模型，我们采用多样性分析、共现网络分析和稳健性测试来阐明乳腺癌对微生物组动态的影响。我们的结果表明，接触乳腺癌会影响细菌群落的组成和结构，时间动态也起了作用。网络分析表明，乳腺癌会破坏微生物相互作用并降低网络复杂性，这可能会损害定殖抗性。此外，网络稳健性分析表明微生物群对节点去除敏感，这表明其对病原体定殖存在潜在易感性。此外，微生物组的预测代谢谱突出了酶EC 6.2.1.2——丁酸盐辅酶A连接酶的重要性，它可能会增加丁酸盐，并平衡定殖抗性的降低。鉴定出 是这种酶的关键贡献者，表明了其在塑造微生物群对乳腺癌反应中的作用。本研究揭示了乳腺癌、肠道微生物组和定殖抗性之间的复杂关系，为乳腺癌患者的潜在治疗策略和诊断方法提供了见解。
