Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, PL-30239 Krakow, Poland.
Biomolecules. 2024 Apr 29;14(5):531. doi: 10.3390/biom14050531.
The adsorption kinetics of human serum albumin (HSA) on bare and poly-L-arginine (PARG)-modified silica substrates were investigated using reflectometry and atomic force microscopy (AFM). Measurements were carried out at various pHs, flow rates and albumin concentrations in the 10 and 150 mM NaCl solutions. The mass transfer rate constants and the maximum protein coverages were determined for the bare silica at pH 4.0 and theoretically interpreted in terms of the hybrid random sequential adsorption model. These results were used as reference data for the analysis of adsorption kinetics at larger pHs. It was shown that the adsorption on bare silica rapidly decreased with pH and became negligible at pH 7.4. The albumin adsorption on PARG-functionalized silica showed an opposite trend, i.e., it was negligible at pH 4 and attained maximum values at pH 7.4 and 150 mM NaCl, the conditions corresponding to the blood serum environment. These results were interpreted as the evidence of a significant role of electrostatic interactions in the albumin adsorption on the bare and PARG-modified silica. It was also argued that our results can serve as useful reference data enabling a proper interpretation of protein adsorption on substrates functionalized by polyelectrolytes.
采用反射测量法和原子力显微镜（AFM）研究了人血清白蛋白（HSA）在裸和聚-L-精氨酸（PARG）修饰的硅基底上的吸附动力学。在 10 和 150 mM NaCl 溶液中，在不同的 pH 值、流速和白蛋白浓度下进行了测量。在 pH 4.0 下确定了裸硅的质量传递速率常数和最大蛋白质覆盖率，并根据混合随机顺序吸附模型进行了理论解释。这些结果被用作在更大 pH 值下分析吸附动力学的参考数据。结果表明，裸硅上的吸附随 pH 值迅速降低，在 pH 7.4 时可忽略不计。PARG 功能化硅上的白蛋白吸附则呈现相反的趋势，即在 pH 4 时可忽略不计，而在 pH 7.4 和 150 mM NaCl 时达到最大值，这是对应于血清环境的条件。这些结果被解释为静电相互作用在裸和 PARG 修饰的硅上吸附白蛋白中的重要作用的证据。还认为，我们的结果可以作为有用的参考数据，有助于对通过聚电解质功能化的基底上的蛋白质吸附进行适当的解释。
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