Puišo Judita, Žvirgždas Jonas, Paškevičius Algimantas, Arslonova Shirin, Adlienė Diana
Department of Physics, Kaunas University of Technology, Studentų Str. 50, LT-51368 Kaunas, Lithuania.
Laboratory of Biodeterioration Research, Institute of Botany, Nature Research Centre, Akademijos Str. 2, LT-08412 Vilnius, Lithuania.
Antibiotics (Basel). 2024 May 14;13(5):441. doi: 10.3390/antibiotics13050441.
Simple low-cost, nontoxic, environmentally friendly plant-extract-based polymer films play an important role in their application in medicine, the food industry, and agriculture. The addition of silver nanoparticles to the composition of these films enhances their antimicrobial capabilities and makes them suitable for the treatment and prevention of infections. In this study, polymer-based gels and films (AgRonPVA) containing silver nanoparticles (AgNPs) were produced at room temperature from fresh red onion peel extract ("Ron"), silver nitrate, and polyvinyl alcohol (PVA). Silver nanoparticles were synthesized directly in a polymer matrix, which was irradiated by UV light. The presence of nanoparticles was approved by analyzing characteristic local surface plasmon resonance peaks occurring in UV-Vis absorbance spectra of irradiated experimental samples. The proof of evidence was supported by the results of XRD and EDX measurements. The diffusion-based method was applied to investigate the antimicrobial activity of several types of microbes located in the environment of the produced samples. Bacteria ATCC 29213, ATCC BAA 747, and ATCC 15442; yeasts CBS 8836 and ATCC 90028; and microscopic fungi assays BTL G-33 and BTL G-38 were used in this investigation. The greatest effect was observed on Staphylococcus aureus, Acinetobacter baumannii, and Pseudomonas aeruginosa bacteria, defining these films as potential candidates for antimicrobial applications. The antimicrobial features of the films were less effective against fungi and the weakest against yeasts.
简单、低成本、无毒、环保的植物提取物基聚合物薄膜在医学、食品工业和农业应用中发挥着重要作用。在这些薄膜的成分中添加银纳米颗粒可增强其抗菌能力，使其适用于感染的治疗和预防。在本研究中，在室温下由新鲜红洋葱皮提取物（“Ron”）、硝酸银和聚乙烯醇（PVA）制备了含银纳米颗粒（AgNPs）的聚合物基凝胶和薄膜（AgRonPVA）。银纳米颗粒直接在聚合物基质中合成，该基质受到紫外线照射。通过分析辐照实验样品的紫外-可见吸收光谱中出现的特征性局域表面等离子体共振峰来确认纳米颗粒的存在。XRD和EDX测量结果支持了这一证据。采用基于扩散的方法研究了所制备样品环境中几种微生物的抗菌活性。本研究使用了细菌ATCC 29213、ATCC BAA 747和ATCC 15442；酵母CBS 8836和ATCC 90028；以及微观真菌检测BTL G-33和BTL G-38。观察到对金黄色葡萄球菌、鲍曼不动杆菌和铜绿假单胞菌的影响最大，这表明这些薄膜是抗菌应用的潜在候选材料。这些薄膜的抗菌特性对真菌的效果较差，对酵母的效果最弱。
